Purification and partial characterization of rat liver pyruvate dehydrogenase kinase activator protein (free pyruvate dehydrogenase kinase)

• Published in: FEBS letters Vol. 308; no. 1; pp. 83 - 86
• Main Authors: Priestman, David A., Mistry, Sharad C., Kerbey, Alan L., Randle, Philip J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.08.1992; Elsevier
• Subjects: adenosine 3′,5′ phosphate; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; cAMP; characterization; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; KAP; kinase activator protein; liver; Mitochondria, Liver - enzymology; Molecular Sequence Data; N-terminal sequence; Oxidoreductases; PDH; PDH-E1; PDH-E2, E1 and E2 components of PDH complex; Protein Kinases - isolation & purification; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases; purification; pyruvate dehydrogenase; Pyruvate dehydrogenase kinase; Rat liver mitochondria; Rats; Specific activity; Starvation; Starvation - enzymology; Specific activity; Starvation; PDH; PDH-E1; N-terminal sequence; Pyruvate dehydrogenase kinase; Rat liver mitochondria; KAP; cAMP; Vertebrata; Mitochondria; Mammalia; Enzymatic activity; Pyruvate dehydrogenase complex; Rat; Enzyme; Liver; Rodentia; N terminal-Sequence
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81056-R

Rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP), a free PDH kinase readily separable from PDH complex and its intrinsic kinase, has been purified to apparent homogeneity from liver mitochondria of fed and 48-h starved rats. On SDS-PAGE an apparently single band of M, 45 kDa was obtained. N-Terminal amino acid sequence analyses (8–10 cycles) confirmed the presence of a single peptide in each case. The specific activity of the purified KAP from 48-h starved rats (14,413 U/mg protein) was 4.5-fold greater than that from fed rats.