Role of glycine-82 as a pivot point during the transition from the inactive to the active form of the yeast Ras2 protein

• Published in: FEBS letters Vol. 281; no. 1; pp. 235 - 239
• Main Authors: Kavounis, Constantin, Verrotti, Arturo C., De Vendittis, Emmanuele, Bozopoulos, Anastasios, Di Blasi, Francesco, Zahn, Regina, Crechet, Jean Bernard, Parmeggiani, Andrea, Tsernoglou, Demetrios, Fasano, Ottavio
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 09.04.1991; Elsevier
• Subjects: [ethylenedinitrilo]tetraacetic acid; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Binding Sites; Biological and medical sciences; EDTA; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - isolation & purification; Fungal Proteins - metabolism; GDP-βS; Glycine; Gpp(NH)p; GTP-Binding Proteins - metabolism; Guanosine diphosphate; Guanosine Diphosphate - metabolism; Guanosine triphosphate; Guanosine Triphosphate - metabolism; guanosine-5′-O-(2-thiodiphosphate); guanyl-5′-yl imidodiphosphate; Guanylyl Imidodiphosphate - metabolism; Miscellaneous; Models, Structural; Mutagenesis, Site-Directed; PAGE; Plasmids; Point mutation; polyacrylamide gel electrophoresis; Protein Conformation; Proteins; Ras; ras Proteins; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Restriction Mapping; S. cerevisae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; SDS; sodium dodecyl sulfate; GDP-βS; Guanosine triphosphate; SDS; Ras; S. cerevisae; Guanosine diphosphate; Gpp(NH)p; Point mutation; PAGE; EDTA
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80401-N

Ras proteins bind either GDP or GTP with high affinity. However, only the GTP-bound form of the yeast Ras2 protein is able to stimulate adenylyl cyclase. To identify amino acid residues that play a role in the conversion from the GDP-bound to the GTP-bound state of Ras proteins, we have searched for single amino acid substitutions that selectively affected the binding of one of the two nucleotides. We have found that the replacement of glycine-82 of the Ras2 protein by serine resulted in an increased rate of dissociation of Gpp(NH)p, a nonhydrolysable analog of GTP, while the GDP dissociation rate was not significantly modified. Glycine-82 resides in a region that is highly conserved between the yeast and human proteins. However, this residue is structurally distant from residues that participate in the binding of the nucleotide, as determined from the crystal structure of the human H- ras gene product. Therefore, the ability of the nucleotide binding site to discriminate between GDP and GTP is dependent not only on residues that are spatially close to the nucleotide, but also on distant amino acids. This is in agreement with the role of glycine-82 as a pivot point during the transition from the GDP- to the GTP-bound form of the Ras proteins.