Budding yeast RSI1/APC2, a novel gene necessary for initiation of anaphase, encodes an APC subunit
SIC1 is a non‐essential gene encoding a CDK inhibitor of Cdc28‐Clb kinase activity. Sic1p is involved in both mitotic exit and the timing of DNA synthesis. To identify other genes involved in controlling Clb‐kinase activity, we have undertaken a genetic screen for mutations which render SIC1 essenti...
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Published in | The EMBO journal Vol. 17; no. 2; pp. 498 - 506 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
15.01.1998
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Subjects | |
Online Access | Get full text |
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Summary: | SIC1 is a non‐essential gene encoding a CDK inhibitor of Cdc28‐Clb kinase activity. Sic1p is involved in both mitotic exit and the timing of DNA synthesis. To identify other genes involved in controlling Clb‐kinase activity, we have undertaken a genetic screen for mutations which render SIC1 essential. Here we describe a gene we have identified by this means, RSI1/APC2. Temperature‐sensitive rsi1/apc2 mutants arrest in metaphase and are unable to degrade Clb2p, suggesting that Rsi1p/Apc2p is associated with the anaphase promoting complex (APC). This is an E3 ubiquitin‐ligase that controls anaphase initiation through degradation of Pds1p and mitotic exit via degradation of Clb cyclins. Indeed, the anaphase block in rsi1/apc2 temperature‐sensitive mutants is overcome by removal of PDS1, consistent with Rsi1p/Apc2p being part of the APC. In addition, like our rsi1/apc2 mutations, cdc23‐1, encoding a known APC subunit, is also lethal with sic1Δ. Thus SIC1 clearly becomes essential when APC function is compromised. Finally, we find that Rsi1p/Apc2p co‐immunoprecipitates with Cdc23p. Taken together, our results suggest that RSI1/APC2 is a subunit of APC. |
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Bibliography: | istex:2B936D80A48AD48B3E24071815F8B734482B9FFC ark:/67375/WNG-P967RV8P-4 ArticleID:EMBJ7590767 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0261-4189 1460-2075 1460-2075 |
DOI: | 10.1093/emboj/17.2.498 |