Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

• Published in: FEBS letters Vol. 223; no. 1; pp. 148 - 154
• Main Authors: Batenburg, A.M., van Esch, J.H., Leunissen-Bijvelt, J., Verkleij, A.J., de Kruijff, B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.10.1987; Elsevier
• Subjects: (dioleoyl-) phosphatidic acid; (dioleoyl-) phosphatidylserine; (dioleoyl-)phosphatidylcholine; (DO)PA; (DO)PC; (DO)PS; 31P-NMR; Artificial membranes and reconstituted systems; Bee Venoms; Biological and medical sciences; Chemical Phenomena; Chemistry, Physical; Freeze Fracturing; Freeze-fracture electron microscopy; Fundamental and applied biological sciences. Psychology; Hexagonal H II phase; Hexagonal HII phase; lipid membranes; Magnetic Resonance Spectroscopy; Melitten; Melittin; Membrane Lipids; Membrane physicochemistry; Models, Biological; Molecular biophysics; Nonbilayer lipid structure; Phosphatidic Acids; phosphatidylglycerol; Phosphatidylglycerols; Phosphatidylserines; phospholipid to peptide molar ratio; Phospholipids; Protein-lipid interaction; R i; Scattering, Radiation; small unilamellar vesicles; SUV; X-Rays; 31P-NMR; Melittin; (DO)PA; Freeze-fracture electron microscopy; SUV; PG; Hexagonal H II phase; Nonbilayer lipid structure; R i; Protein-lipid interaction; (DO)PS; (DO)PC; Diphosphatidylglycerol; Peptides; Dispersed state; Liposome; Phosphatidylglycerol; Molecular interaction; Artificial membrane; NMR spectrometry; Electron microscopy; Phase transitions
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(87)80526-4

A characterization of the structural alterations induced by melittin in model-membranes of dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on the use of 31P-NMR, freeze-fracture electron microscopy and small angle X-ray scattering. In accordance with earlier findings on the cardiolipinmelittin system, melittin is found to have an inverted phase inducing effect on these negatively charged lipids, in contrast to the influence on zwitterionic phospholipids. In phosphatidic acid this is expressed in the formation of an H II phase; in phosphatidylglycerol a less ordered, non-lamellar structure with low water content is adopted.