Metabolism of fatty acid in yeast: Characterisation of β-oxidation and ultrastructural changes in the genus Sporidiobolus sp. cultivated on ricinoleic acid methyl ester

• Published in: FEMS microbiology letters Vol. 250; no. 1; pp. 63 - 69
• Main Authors: Feron, Gilles, Blin-Perrin, Caroline, Krasniewski, Isabelle, Mauvais, Geneviève, Lherminier, Jeannine
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.09.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press; Wiley-Blackwell
• Subjects: 3-Hydroxyacyl CoA Dehydrogenases - metabolism; Acyl-CoA oxidase; Acyl-CoA Oxidase - metabolism; Acyltransferases - metabolism; Basidiomycota - growth & development; Basidiomycota - metabolism; Basidiomycota - ultrastructure; Biological and medical sciences; Catalase; Catalase - metabolism; Cell Compartmentation; Cultivation; Cytology; Enoyl-CoA hydratase; Enoyl-CoA Hydratase - metabolism; Esters; Fatty acids; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Kinetics; Life Sciences; Microbiology; Microbiology and Parasitology; Microscopy, Electron; Mitochondria; Mitochondria - metabolism; Mitochondria - ultrastructure; Mitochondrion; Mycological methods and techniques used in mycology; Mycology; Oxidation; Oxidation-Reduction; Ricinoleic acid; Ricinoleic Acids - metabolism; Species Specificity; Sporidiobolus genus; Thiolase; Yeast; Yeast β-oxidation; Yeasts; γ-Decalactone; Mitochondrion; γ-Decalactone; Sporidiobolus genus; Ricinoleic acid; Yeast β-oxidation; genus; Yeast; Basidiomycetes; Lipids; Metabolism; Fatty acids; Fungi; Characterization; Mitochondria; Microscopy; Sporidiobolus; β Oxidation; Thallophyta; MITOCHONDRION; SPORIDIOBOLUS GENUS; RICINOLEIC ACID; YEAST BETA-OXIDATION; GAMMA-DECALACTONE
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.06.045

Cell structure modifications and β-oxidation induction were monitored in two strains of Sporidiobolus, Sp. Ruinenii and Sp. pararoseus after cultivation on ricinoleic acid methyl ester. Ultrastructural observations of the yeast before and after cultivation on fatty acid esters did not reveal major modifications in Sp. ruinenii. Unexpectedly, in Sp. pararoseus a proliferation of the mitochondrion was observed. After induction, Sp. ruinenii principally exhibited an increase in the activities of acyl-CoA oxidase (ACO), hydroxyacyl-CoA deshydrogenase (HAD), thiolase and catalase. In contrast, Sp. pararoseus lacked ACO and catalase activities, but an increase in acyl-CoA deshydrogenase (ACDH) and enoyl-CoA hydratase (ECH) activity was observed. These data suggest that in Sp. ruinenii, β-oxidation is preferentially localized in the microbody, whereas in Sp. pararoseus it might be localized in the mitochondria.