Binding of actinomycin D to DNA revealed by DNase I footprinting

We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleoti...

Full description

Saved in:
Bibliographic Details
Published inFEBS letters Vol. 164; no. 1; pp. 97 - 101
Main Authors Scamrov, A.V., Beabealashvilli, R.Sh
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 28.11.1983
Elsevier
Subjects
Actinomycin D
Base Sequence
Biological and medical sciences
Chemical Phenomena
Chemistry
cooperativity
Dactinomycin
Deoxyribonuclease I
DNA
DNA-ligand interaction
DNase I
Endodeoxyribonucleases - metabolism
Footprint
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Molecular biophysics
Plasmids
Promoter lac UV5
Substrate Specificity
Actinomycin D
Footprint
DNase I
DNA-ligand interaction
Promoter lac UV5
Molecular association
Binding site
Deoxyribonuclease I
DNA
Nucleotide fragment
Online AccessGet full text

Cover

More Information
Summary:We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X ≠ G and Y ≠ C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80027-1