Centrosome amplification in bladder washing cytology specimens is a useful prognostic biomarker for non-muscle invasive bladder cancer

• Published in: Cancer genetics Vol. 206; no. 1; pp. 12 - 18
• Main Authors: Miyachika, Yoshihiro, Yamamoto, Yoshiaki, Matsumoto, Hiroaki, Nishijima, Jun, Kawai, Yoshihisa, Nagao, Kazuhiro, Hara, Takahiko, Sakano, Shigeru, Matsuyama, Hideyasu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2013
• Subjects: Aged; Aged, 80 and over; Antibodies; biomarker; Biomarkers, Tumor - analysis; bladder washing cytology (BWC); Carcinoma - diagnosis; Carcinoma - pathology; Centrosome - metabolism; Centrosome - physiology; Centrosome amplification (CA); Cohort Studies; Cytodiagnosis; disease progression; Female; Hematology, Oncology and Palliative Medicine; Humans; Male; Medical Education; Middle Aged; Neoplasm Invasiveness; non-muscle invasive bladder cancer (NMIBC); Predictive Value of Tests; Prognosis; Urinary Bladder - pathology; Urinary Bladder - ultrastructure; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - pathology; bladder washing cytology (BWC); Centrosome amplification (CA); disease progression; non-muscle invasive bladder cancer (NMIBC); biomarker
• ISSN: 2210-7762; 2210-7770
• DOI: 10.1016/j.cancergen.2012.11.004

We investigated whether centrosome amplification (CA) obtained from bladder washing cytology (BWC) specimens may be a useful prognostic biomarker for patients with non-muscle invasive bladder cancer (NMIBC). The study cohort included 78 patients with pathologically confirmed NMIBC. BWC specimens were obtained from all patients during transurethral resection of bladder tumor (TURBT), and CA was evaluated by immunofluorescence staining using a pericentrin polyclonal antibody. A positive case of CA was defined as a specimen in which >5% of cells contained ≥3 centrosomes per cell. CA was detected in 26.9% (21 of 78) of BWC specimens obtained from NMIBC patients. Disease progression was observed in 11.5% (9 of 78) of patients, with a median follow-up of 32 months. In univariate analyses, CA obtained from BWC specimens, initial or recurrent, and washing cytology were significantly associated with progression-free survival ( P = 0.009, 0.02, and 0.03, respectively). Multivariate Cox model analyses revealed that CA was the most significant prognostic factor for disease progression (hazard ratio: 2.22, 95% confidence interval: 1.13–4.90, P = 0.022). These data suggest that analysis of CA using bladder washing cytological specimens may provide crucial predictive information regarding disease progression in NMIBC.