3D Multicellular Tumor Spheroids in a Microfluidic Droplet System for Investigation of Drug Resistance

A three-dimensional (3D) tumor spheroid model plays a critical role in mimicking tumor microenvironments in vivo. However, the conventional culture methods lack the ability to manipulate the 3D tumor spheroids in a homogeneous manner. To address this limitation, we developed a microfluidic-based dro...

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Published inPolymers Vol. 14; no. 18; p. 3752
Main Authors Lee, Sang Ik, Choi, Yoon Young, Kang, Seong Goo, Kim, Tae Hyeon, Choi, Ji Wook, Kim, Young Jae, Kim, Tae-Hyung, Kang, Taewook, Chung, Bong Geun
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2022
MDPI
Subjects
anti-cancer drug screening
Biocompatibility
Breast cancer
Cancer
Cell culture
concentration gradient generator
Diagnosis
Droplets
Drug resistance
Drug therapy
Fibroblasts
In vivo methods and tests
Investigations
Medical research
microdroplet
Microfluidics
multicellular cancer spheroid
Screening
Silicon wafers
Simulation
Spheroids
Three dimensional models
Toxicity
Tumors
Velocity
South Korea
Japan
St Louis Missouri
United States > US
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Summary:A three-dimensional (3D) tumor spheroid model plays a critical role in mimicking tumor microenvironments in vivo. However, the conventional culture methods lack the ability to manipulate the 3D tumor spheroids in a homogeneous manner. To address this limitation, we developed a microfluidic-based droplet system for drug screening applications. We used a tree-shaped gradient generator to control the cell density and encapsulate the cells within uniform-sized droplets to generate a 3D gradient-sized tumor spheroid. Using this microfluidic-based droplet system, we demonstrated the high-throughput generation of uniform 3D tumor spheroids containing various cellular ratios for the analysis of the anti-cancer drug cytotoxicity. Consequently, this microfluidic-based gradient droplet generator could be a potentially powerful tool for anti-cancer drug screening applications.
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These authors contributed equally to this work.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym14183752