The involvement of FAK-PI3K-AKT-Rac1 pathway in porcine reproductive and respiratory syndrome virus entry

• Published in: Biochemical and biophysical research communications Vol. 458; no. 2; pp. 392 - 398
• Main Authors: Ni, Bo, Wen, Li-Bin, Wang, Rui, Hao, Hong-Ping, Huan, Chang-Chao, Wang, Xin, Huang, Li, Miao, Jin-Feng, Fan, Hong-Jie, Mao, Xiang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.03.2015
• Subjects: Animals; FAK; Focal Adhesion Kinase 1 - metabolism; Infection; Oncogene Protein v-akt - metabolism; Phosphatidylinositol 3-Kinases - metabolism; Phosphotransferases - metabolism; Porcine Reproductive and Respiratory Syndrome - enzymology; Porcine Reproductive and Respiratory Syndrome - virology; Porcine reproductive and respiratory syndrome virus; Porcine respiratory and reproductive syndrome virus; Porcine respiratory and reproductive syndrome virus - physiology; Rac1; rac1 GTP-Binding Protein - metabolism; Signal Transduction; Swine - metabolism; Swine - virology; Virus Internalization; Infection; FAK; Porcine reproductive and respiratory syndrome virus; Rac1
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2015.01.126

CD163 and sialoadhesin had been reported as the two receptors for porcine reproductive and respiratory syndrome virus (PRRSV) infection. The signaling pathway activated by PRRSV entry was seldom reported. In our studies, we demonstrated that PRRSV entry triggers FAK, PI3K, AKT and Rac1 activation. The signaling pathway FAK-PI3K-AKT-Rac1 is essential for PRRSV entry. Blocking FAK by PF573228 attenuates the activation of PI3K, AKT, Rac1 and the cytoskeleton remodeling induced by virus entry. Inhibitors to FAK, PI3K, AKT and Rac1 can significantly inhibit the virus entry. In conclusion, our observations reveal that PRRSV triggers the activation of FAK-PI3K-AKT-Rac1 signaling pathway to facilitate its entry into cells. •PRRSV entry triggers FAK, PI3K, AKT and Rac1 activation.•The signaling pathway FAK-PI3K-AKT-Rac1 is essential for PRRSV entry.•FAK, PI3K, AKT and Rac1 inhibitors affect PRRSV entry.