Characterization of Fructan Metabolism During Jerusalem Artichoke ( Helianthus tuberosus L.) Germination

• Published in: Frontiers in plant science Vol. 9; p. 1384
• Main Authors: Jiao, Jiao, Wang, Ji, Zhou, Mengjia, Ren, Xuyang, Zhan, Wenyue, Sun, Zongjiu, Zhao, Haiyan, Yang, Yao, Liang, Mingxiang, den Ende, Wim Van
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 19.09.2018
• Subjects: enzyme activity; gene expression; jerusalem artichoke; Plant Science; sugar; tuber sprouting; jerusalem artichoke; tuber sprouting; sugar; enzyme activity; gene expression
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2018.01384

The inulin-type fructans in Jerusalem artichoke ( L.) tubers exhibit different degrees of polymerization and are critical for germination. We aimed to characterize the sugar metabolism dynamics in the tubers without bud eyes or shoots (T) and BE/S of indoor- and field-grown Jerusalem artichokes during germination. and (1-fructan exohydrolases II and III, inulin-degrading enzymes) expression increased 5 days after planting indoors, whereas expression increased 72 days after planting in the field in T and BE/S. (sucrose:sucrose 1-fructosyl transferase, inulin synthesis initiator), and (fructan:fructan 1-fructosyl transferase, inulin elongator) expression generally decreased in indoor-grown T. The enzyme activities of 1-FEH and 1-FFT were unchanged during germination in both indoor- and field-grown T and BE/S, whereas 1-SST activity decreased in indoor-grown T, while 1-FEH and 1-FFT activities increased as a function of germination time in BE/S of both indoor- and field-grown tubers. The total soluble sugar content gradually decreased in T after germination indoors or in the field, while at the end of germination, the sucrose and fructan contents decreased, and fructose content increased in the field. The enzyme activities of soluble vacuolar (VI) or neutral invertase (NI) did not change significantly, except at the late germination stage. Sucrose synthase (SS) and sucrose-phosphate synthase (SPS) activities were not significantly changed in T and BE/S in indoor-grown artichokes, while SS activity gradually increased, and SPS activity gradually decreased in field-grown artichokes, alongside sucrose degradation. Compared to T, BE/S generally had higher enzyme activities of 1-FEH and 1-FFT, promoting inulin hydrolysis. This work shows that the process of tuber germination is similar indoors and in the field, and germination studies can therefore be conducted in either environment.