Isolation and Role of PmRGL2 in GA-mediated Floral Bud Dormancy Release in Japanese Apricot ( Prunus mume Siebold et Zucc.)

• Published in: Frontiers in plant science Vol. 9; p. 27
• Main Authors: Lv, Lin, Huo, Ximei, Wen, Luhua, Gao, Zhihong, Khalil-Ur-Rehman, Muhammad
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 26.01.2018
• Subjects: DELLA; dormancy; GA4; gibberellins; Plant Science; RGL2; SLY1; Japanese apricot; dormancy; SLY1; gibberellins; GA4; RGL2; DELLA
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2018.00027

Bud dormancy release is regulated by gibberellins (GAs). DELLA proteins are highly conserved and act as negative regulators in GA signaling pathway. The present study established a relationship between in Japanese apricot and GA levels during dormancy release of floral buds. Overexpression of in poplar delayed the onset of bud dormancy and resulted in dwarf plants, relative to wild-type trees. exhibited higher expression during ecodormancy and relatively lower expression during endodormancy. The relative level of GA exhibited an increasing trend at the transition from endodormancy to ecodormancy and displayed a similar expression pattern of genes related to GA metabolism, , , in both Japanese apricot and transgenic poplar. These results suggests that acts as an integrator and negative regulator of dormancy via a GA-signaling pathway. Moreover, an interaction between and SLY1 in a yeast two hybrid (Y2H) system further suggests that SCF E3 ubiquitin ligases, such as , may be a critical factor in the regulation of through an SCF -proteasome pathway. Our study demonstrated that plays a negative role in bud dormancy release by regulating the GA biosynthetic enzymes, and and the GA receptor, .