Heavy metal resistance in Arthrobacter ramosus strain G2 isolated from mercuric salt-contaminated soil
Present study describes isolation of a multiple metal-resistant Arthrobacter ramosus strain from mercuric salt-contaminated soil. The isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. Maximum tolerated concentrations for above metals w...
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Published in | Journal of hazardous materials Vol. 177; no. 1; pp. 481 - 486 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier B.V
15.05.2010
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Present study describes isolation of a multiple metal-resistant
Arthrobacter ramosus strain from mercuric salt-contaminated soil. The isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. Maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369
μM, respectively. The isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bioremediation of heavy metal-contaminated sites. Chromate reductase and mercuric reductase (MerA) activities in protein extract of the culture were found to be 2.3 and 0.17
units
mg
−1 protein, respectively. MerA enzyme was isolated from the culture by (NH
4)
2SO
4 precipitation followed by dye affinity chromatography and its identity was confirmed by nano-LC–MS/MS. Its monomeric molecular weight, and optimum pH and temperature were 57
kDa, 7.4 and 55
°C, respectively. Thus, the enzyme was mildly thermophilic as compared to other MerA enzymes.
K
m and
V
max of the enzyme were 16.9
μM HgCl
2 and 6.2
μmol
min
−1
mg
−1 enzyme, respectively. The enzyme was found to be NADPH-specific. To our knowledge this is the first report on characterization of MerA enzyme from an
Arthrobacter sp. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0304-3894 1873-3336 |
DOI: | 10.1016/j.jhazmat.2009.12.058 |