The expression profile of ATP-binding cassette transporter genes in breast carcinoma

• Published in: Pharmacogenomics Vol. 14; no. 5; pp. 515 - 529
• Main Authors: Hlavá, Viktor, Brynychová, Veronika, Václavíková, Radka, Ehrlichová, Marie, Vrána, David, Pecha, Václav, Ko evnikovová, Renata, Trnková, Markéta, Gat k, Ji í, Kopperová, Dana, Gut, Ivan, Sou ek, Pavel
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.04.2013
• Subjects: ABC transporters; Antineoplastic Agents - administration & dosage; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Breast cancer; breast carcinoma; Breast Neoplasms - drug therapy; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Carcinoma - drug therapy; Carcinoma - genetics; Carcinoma - pathology; Carrier proteins; expression; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes; Genetic aspects; Genetic Association Studies; Humans; Middle Aged; Neoadjuvant Therapy; Physiological aspects; prognosis; resistance; response; Czech Republic
• ISSN: 1462-2416; 1744-8042
• DOI: 10.2217/pgs.13.26

ATP-binding cassette (ABC) transporters contribute to development of resistance to anticancer drugs via ATP-dependent drug efflux. A major goal of our study was to investigate associations between the expression of ABC transporters and outcome of breast carcinoma patients. Transcript levels of all 49 human ABC transporters were determined in post-treatment tumor and non-neoplastic tissue samples from 68 breast carcinoma patients treated by neoadjuvant chemotherapy. Six ABC transporters were then evaluated in independent series of 100 pretreatment patients. / / / / , / / , / , / , and were significantly downregulated and / / / , / / / / , / / / / / , / , , / / and were upregulated in post-treatment tumors compared with non-neoplastic tissues. Significant associations of intratumoral levels of and with grade and expression of hormonal receptors were found in both sets of patients. , and levels were significantly associated with the response to neoadjuvant chemotherapy in post-treatment patients. Protein expression of ABCA12, ABCC8 and ABCD2 in tumor tissues of patients with breast carcinoma was observed by immunoblotting for the first time. , , , and present potential modifiers of progression and response to the chemotherapy of breast carcinoma. Original submitted 27 July 2012; Revision submitted 5 February 2013