Dioxin induces transforming growth factor-alpha in human keratinocytes

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a widespread environmental toxicant, is a tumor promoter that induces hyperplasia in epithelial cells. Exposure of cultured human keratinocytes to TCDD, resulted in a time-dependent dioxin-specific Ah receptor-mediated release of transforming growth factor...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 266; no. 15; pp. 9591 - 9597
Main Authors Choi, E.J. (University of Minnesota, Minneapolis, MN), Toscano, D.G, Ryan, J.A, Riedell, N, Toscano, W.A. Jr
Format Journal Article
LanguageEnglish
Published Bethesda, MD American Society for Biochemistry and Molecular Biology 25.05.1991
Subjects
Biological and medical sciences
Blotting, Northern
Blotting, Western
Carcinogens
Cell physiology
COMPOSE ORGANOCHLORE
COMPUESTO ORGANICO DEL CLORO
CRECIMIENTO
CROISSANCE
CULTIVO DE CELULAS
CULTURE DE CELLULE
Epidermal Growth Factor - metabolism
ErbB Receptors - metabolism
Fundamental and applied biological sciences. Psychology
HIDROCARBUROS
HORMONAS
HORMONE
Humans
HYDROCARBURE
Keratinocytes - metabolism
Molecular and cellular biology
Phosphorylation
Polychlorinated Dibenzodioxins - toxicity
Responses to growth factors, tumor promotors, other factors
RNA, Messenger - analysis
TCDD
TOXICIDAD
TOXICITE
transforming growth factor alpha
Transforming Growth Factor alpha - metabolism
Human
Regulation(control)
Cell line
Induction
Secretion
Toxicity
Transforming growth factor
Blotting assay
Epithelial cell
Keratinocyte
Biological receptor
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Summary:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a widespread environmental toxicant, is a tumor promoter that induces hyperplasia in epithelial cells. Exposure of cultured human keratinocytes to TCDD, resulted in a time-dependent dioxin-specific Ah receptor-mediated release of transforming growth factor-alpha (TGF-alpha) into the culture medium. Cultures exposed to TCDD showed a rate of TGF-alpha secretion into the medium of about 30 fmol/ml/day, as well as a 3- to 6-fold increase in TGF-alpha mRNA expression. Increased production of TGF-alpha in human keratinocytes exposed to TCDD demonstrates a modulation of autocrine regulation in those cells. These results suggest that induction of TGF-alpha could be an important part of the mechanism of dioxin-mediated toxicity and tumor promotion
Bibliography:H01
9161544
T10
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ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(18)92861-9