Simultaneously enhanced stability and biological activities of chlorogenic acid by covalent grafting with soluble oat β-glucan

• Published in: Food Chemistry: X Vol. 17; p. 100546
• Main Authors: Luo, Yan, Li, Yun-Cheng, Meng, Fan-Bing, Wang, Zheng-Wu, Liu, Da-Yu, Chen, Wei-Jun, Zou, Long-Hua
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30.03.2023; Elsevier
• Subjects: Antibacterial activities; Antioxidant activities; Chlorogenic acid; Grafting modification; Stability; Water-soluble oat β-glucan; Water-soluble oat β-glucan; Grafting modification; Chlorogenic acid; Antioxidant activities; Antibacterial activities; Stability
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2022.100546

•The grafting of CA onto OβGH was achieved by an EDC/NHS coupling method.•The Structure properties of CA-OβGH were analyzed by FT-IR, UV–vis, NMR and X-ray.•The storage stability and antioxidant activities of CA-OβGH significantly improved.•CA-OβGH showed excellent antibacterial activities against foodborne pathogens. Chlorogenic acid (CA) has a wide range of biological activities but the chemical structure is extremely unstable. In this study, CA was grafted onto a soluble oat β-glucan (OβGH) to improve the stability. Although the crystallinity and thermal stability of CA-OβGH conjugates reduced, the storage stability of CA significantly improved. The DPPH and ABTS scavenging ability of CA-OβGH IV (graft ratio 285.3 mg CA/g) were higher than 90 %, which is closed to activities of equivalent concentration of Vc (93.42 %) and CA (90.81 %). The antibacterial abilities of CA-OβGH conjugates are improved compared to the equivalent content of CA and potassium sorbate. Particularly, the inhibition rate of CA-OβGH for gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes) are significantly higher than that of gram-negative bacteria (Escherichia coli). The results demonstrated that covalent grafted CA with soluble polysaccharide is an effective strategy to enhance its stability and biological activities.