Regulation of Sertoli Cell Number and Activity by Follicle-Stimulating Hormone and Androgen during Postnatal Development in the Mouse

• Published in: Endocrinology (Philadelphia) Vol. 145; no. 1; pp. 318 - 329
• Main Authors: Johnston, Heather, Baker, Paul J, Abel, Margaret, Charlton, Harry M, Jackson, Gary, Fleming, Lynne, Kumar, T. Rajendra, O’Shaughnessy, Peter J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.01.2004; Oxford University Press
• Subjects: Androgen receptors; Androgens; Androgens - metabolism; Animals; Biological and medical sciences; Birth; Cell growth; Cell number; Cell proliferation; Cryptorchidism; Female; Fetuses; Follicle Stimulating Hormone, beta Subunit - genetics; Follicle Stimulating Hormone, beta Subunit - metabolism; Follicle-stimulating hormone; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Developmental; Male; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Neonates; Postpartum period; Receptor mechanisms; Receptors; Receptors, Androgen - genetics; Receptors, FSH - genetics; RNA, Messenger - analysis; Sertoli Cells - cytology; Sertoli Cells - metabolism; Testis - cytology; Testis - growth & development; Testis - metabolism; Testosterone; Vertebrates: endocrinology; Sertoli cell; Androgen; Rodentia; Gonadotropin; Testicle; Male genital system; Postnatal development; Vertebrata; Mammalia; Adenohypophyseal hormone; Mouse; Animal; Sex steroid hormone; Follicle stimulating hormone
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2003-1055

The roles of FSH and androgen in the postnatal development of Sertoli cell number and function have been investigated using mice that lack FSH (FSHβKO), FSH-receptors (FSHRKO), or androgen receptors (Tfm). At birth and d 5, Sertoli cell number was normal in FSHRKO and FSHβKO mice, but was significantly reduced on d 20 and in adulthood. In contrast, Sertoli cell number was reduced at birth in Tfm mice and remained significantly less than normal up to adulthood. Sertoli cell activity was determined through measurement of 11 different mRNA transcript levels. From birth to adulthood, the expression of most transcripts increased, with a significant rise occurring between d 5 and 10. In animals lacking FSH stimulation, mRNA expression (measured per Sertoli cell) was largely normal on d 5, but was reduced in seven transcripts on d 20 and in five transcripts at adulthood. In Tfm mice two transcripts showed reduced expression on d 5, and four were reduced on d 20, although expression in adult Tfm mice did not differ from that in normal cryptorchid controls. The results show that 1) testosterone, but not FSH, is required for Sertoli cell proliferation during fetal and early neonatal life; 2) FSH and testosterone both regulate the late stages of Sertoli cell proliferation; 3) FSH has a general trophic effect on Sertoli cell activity in the pubertal and adult mouse; and 4) androgens are required for specific transcript expression during prepubertal development. Specific effects of androgens were not seen in the adult, although these may be masked by the effects of cryptorchidism.