Survival of transfused CD18-positive granulocytes and their chemiluminescent response in a heifer with leukocyte adhesion deficiency

• Published in: Journal of Veterinary Medical Science Vol. 60; no. 2; pp. 261 - 262
• Main Authors: Nagahata, H. (College of Dairying, Ebetsu, Hokkaido (Japan)), Sakurai, N, Matsuki, S, Miura, T, Nioka, K, Kociba, G.J
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.02.1998; Japan Science and Technology Agency
• Subjects: Animals; Biomarkers; BLAD; BOVIN; CATTLE; Cattle Diseases; CD18 Antigens - blood; CD18-positive cell; Cell Survival; DEFICIENCY DISEASES; ENFERMEDADES CARENCIALES; Female; GANADO BOVINO; GENISSE; GRANULOCITOS; GRANULOCYTE; granulocyte transfusion; GRANULOCYTES; HEIFERS; LEUCOCITOS; LEUCOCYTE; Leukocyte Transfusion - veterinary; Leukocyte-Adhesion Deficiency Syndrome - therapy; Leukocyte-Adhesion Deficiency Syndrome - veterinary; LEUKOCYTES; Luminescent Measurements; MALADIE DE CARENCE; Neutrophils - cytology; Neutrophils - immunology; Neutrophils - physiology; NOVILLA
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.60.261

Granulocyte transfusion (GT) was performed in an 8-month-old heifer with leukocyte adhesion deficiency (BLAD) to monitor the changes in transfused CD18-positive neutrophils and associated neutrophil chemiluminescent (CL) response in β2-integrin-deficient host. The CD18-positive neutrophils were detected in blood from the BLAD heifer during the first 3 hr after 2.6 × 109 cells were infused by GT, and disappeared by 5 hr after GT. The CL response of neutrophils was increased 1.7 to 2.8-fold in the BLAD heifer during the first 3 hr after GT, thereafter CL response decreased gradually from 2 to 5 hr after GT.