A Novel G16B09-Like Effector From Heterodera avenae Suppresses Plant Defenses and Promotes Parasitism

• Published in: Frontiers in plant science Vol. 10; p. 66
• Main Authors: Yang, Shanshan, Dai, Yiran, Chen, Yongpan, Yang, Jun, Yang, Dan, Liu, Qian, Jian, Heng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 08.02.2019
• Subjects: effector; ETI; G16B09 family; Heterodera avenae; Plant Science; PTI; suppressed plant defense; Heterodera avenae; effector; ETI; PTI; G16B09 family; suppressed plant defense
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2019.00066

Plant parasitic nematodes secrete effectors into host plant tissues to facilitate parasitism. In this study, we identified a G16B09-like effector protein family from the transcriptome of , and then verified that most of the members could suppress programmed cell death triggered by BAX in . Ha18764, the most homologous to G16B09, was further characterized for its function. Our experimental evidence suggested that was specifically expressed in the dorsal gland and was dramatically upregulated in the J4 stage of nematode development. A secretion system in barley showed that the signal peptide of Ha18764 had secretion activity to deliver mCherry into plant cells. overexpressing or was more susceptible to . In contrast, BSMV-based host-induced gene silencing (HIGS) targeting attenuated parasitism and its reproduction in wheat plants. Transient expression of suppressed PsojNIP, Avr3a/R3a, RBP-1/Gpa2, and MAPK kinases (MKK1 and NPK1 )-related cell death in . Co-expression assays indicated that Ha18764 also suppressed cell death triggered by four putative cell-death-inducing effectors. Moreover, Ha18764 was also shown strong PTI suppression such as reducing the expression of plant defense-related genes, the burst of reactive oxygen species, and the deposition of cell wall callose. Together, our results indicate that Ha18764 promotes parasitism, probably by suppressing plant PTI and ETI signaling in the parasitic stages of .