Immunization with hepatitis C virus-like particles results in control of hepatitis C virus infection in chimpanzees

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 104; no. 20; pp. 8427 - 8432
• Main Authors: Elmowalid, Gamal A, Qiao, Ming, Jeong, Sook-Hyang, Borg, Brian B, Baumert, Thomas F, Sapp, Ronda K, Hu, Zongyi, Murthy, Krishna, Liang, T. Jake
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 15.05.2007; National Acad Sciences
• Subjects: Adjuvants, Immunologic; Animals; Antibody Formation - immunology; Antigens; Biological Sciences; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; Cell Proliferation; Dosage; Hepacivirus - immunology; Hepatitis C - prevention & control; Hepatitis C virus; Immune response; Immunization; Infections; Liver; Liver - cytology; Liver - immunology; Pan troglodytes; Pan troglodytes - immunology; T lymphocytes; Time Factors; Vaccination; Vaccines - immunology; Viremia; Viremia - immunology; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0702162104

Recombinant hepatitis C virus (HCV)-like particles (HCV-LPs) containing HCV structural proteins (core, E1, and E2) produced in insect cells resemble the putative HCV virions and are capable of inducing strong and broad humoral and cellular immune responses in mice and baboons. Here, we present evidence on the immunogenicity and induction of protective immunity by HCV-LPs in chimpanzees. Chimpanzees (two in each group), were immunized with HCV-LPs or HCV-LPs plus AS01B adjuvant. After immunizations, all animals developed an HCV-specific immune response including IFN-γ⁺, IL-2⁺, CD4⁺, and CD8⁺ T cell and proliferative lymphocyte responses against core, E1, and E2. Upon challenge with an infectious HCV inoculum, one chimpanzee developed transient viremia with low HCV RNA titers (10³ to 10⁴ copies per ml) in the third and fourth weeks after the challenge. The three other chimpanzees became infected with higher levels of viremia (10⁴ to 10⁵ copies per ml), but their viral levels became unquantifiable (<10³ copies per ml) 10 weeks after the challenge. After the HCV challenge, all four chimpanzees demonstrated a significant increase in peripheral and intrahepatic T cell and proliferative responses against the HCV structural proteins. These T cell responses coincided with the fall in HCV RNA levels. Four naïve chimpanzees were infected with the same HCV inoculum, and three developed persistent infection with higher viremia in the range of 10⁵ to 10⁶ copies per ml. Our study suggests that HCV-LP immunization induces HCV-specific cellular immune responses that can control HCV challenge in the chimpanzee model.