Partially Oxygenated Sickled Cells: Sickle-Shaped Red Cells Found in Circulating Blood of Patients with Sickle Cell Disease

A previously uncharacterized type of sickled cell was found in venous blood of patients with sickle cell disease when blood was collected without exposure to air and fixed immediately with 1% glutaraldehyde solution equilibrated with 5% oxygen. These cells were either elongated, resembling irreversi...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 91; no. 26; pp. 12589 - 12593
Main Authors Asakura, Toshio, Mattiello, Julian A., Obata, Kazuo, Asakura, Kenji, Reilly, Michael P., Tomassini, Natale, Schwartz, Elias, Ohene-Frempong, Kwaku
Format Journal Article
LanguageEnglish
Published United States National Academy of the Sciences of the United States of America 20.12.1994
National Acad Sciences
National Academy of Sciences
Subjects
Anemia, Sickle Cell - blood
Blood
Cellular biology
Deoxygenation
Erythrocytes
Erythrocytes, Abnormal - pathology
Hemoglobins
Humans
Light
man
Medical research
Medical syringes
Microscopy, Electron
Oxygen
Oxygen - blood
Polymers
Scattering, Radiation
Sickle cell anemia
Sickle cell disease
Sickles
Tissue oxygenation
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Summary:A previously uncharacterized type of sickled cell was found in venous blood of patients with sickle cell disease when blood was collected without exposure to air and fixed immediately with 1% glutaraldehyde solution equilibrated with 5% oxygen. These cells were either elongated, resembling irreversibly sickled cells (ISCs), or nonelongated, with a raisinlike shape. Both types assumed a normal discoidal shape upon full oxygenation. Since these cells exist only under partially oxygenated conditions, they are described as partially oxygenated sickled cells (POSCs). POSCs are morphologically distinct from partially deoxygenated sickled cells formed during deoxygenation by having rounded edges, while the latter have sharp edges. Transmission electron microscopy of POSCs revealed various amounts of misaligned Hb S polymers. Investigations in vitro demonstrated the formation of POSC-like cells by partial oxygenation of deoxygenated cells. Since POSCs contain intracellular fibers and sickle readily upon deoxygenation, they may have clinical and pathological significance.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.26.12589