An ethanol extract of Artemisia iwayomogi activates PPARδ leading to activation of fatty acid oxidation in skeletal muscle

• Published in: PloS one Vol. 7; no. 3; p. e33815
• Main Authors: Cho, Si Young, Jeong, Hyun Woo, Sohn, Jong Hee, Seo, Dae-Bang, Kim, Wan Gi, Lee, Sang-Jun
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.03.2012; Public Library of Science (PLoS)
• Subjects: Activation; Animals; Artemisia - chemistry; Biology; Carnitine; Cholesterol; Dehydrogenases; Diabetes; Diet, High-Fat - adverse effects; Efflux; Energy expenditure; Ethanol; Ethanol - chemistry; Fatty acids; Fatty Acids - metabolism; Gene expression; Gene Expression Regulation - drug effects; Genes; Glucose; Glucose - metabolism; High fat diet; Humans; Insulin; Kinases; Kinetics; Ligands; Lipid metabolism; Lipids; Male; Medicine; Metabolic syndrome; Metabolism; Mice; Mice, Inbred C57BL; Mode of action; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Obesity; Obesity - drug therapy; Obesity - metabolism; Oxidation; Oxidation-Reduction - drug effects; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; PPAR delta - metabolism; Protein Binding; Proteins; Pyruvic acid; R&D; Research & development; Rodents; Skeletal muscle; Transcriptional Activation - drug effects; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0033815

Although Artemisia iwayomogi (AI) has been shown to improve the lipid metabolism, its mode of action is poorly understood. In this study, a 95% ethanol extract of AI (95EEAI) was identified as a potent ligand of peroxisome proliferator-activated receptorδ (PPARδ) using ligand binding analysis and cell-based reporter assay. In cultured primary human skeletal muscle cells, treatment of 95EEAI increased expression of two important PPARδ-regulated genes, carnitine palmitoyl-transferase-1 (CPT1) and pyruvate dehydrogenase kinase isozyme 4 (PDK4), and several genes acting in lipid efflux and energy expenditure. Furthermore, 95EEAI stimulated fatty acid oxidation in a PPARδ-dependent manner. High-fat diet-induced obese mice model further indicated that administration of 95EEAI attenuated diet-induced obesity through the activation of fatty acid oxidation in skeletal muscle. These results suggest that a 95% ethanol extract of AI may have a role as a new functional food material for the prevention and/or treatment of hyperlipidermia and obesity.