Noninvasive Measurement of Liver Fibrosis Using Transient Elastography in Pediatric Patients with Major Thalassemia Who Are Candidates for Hematopoietic Stem Cell Transplantation

• Published in: Biology of blood and marrow transplantation Vol. 20; no. 12; pp. 1912 - 1917
• Main Authors: Hamidieh, Amir Ali, Shazad, Babak, Ostovaneh, Mohammad Reza, Behfar, Maryam, Tayebi, Sirous, Malekzadeh, Reza, Ghavamzadeh, Ardeshir, Poustchi, Hossein
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2014
• Subjects: beta-Thalassemia - pathology; beta-Thalassemia - therapy; Child; Child, Preschool; Elasticity Imaging Techniques - methods; Female; Hematology, Oncology and Palliative Medicine; Hematopoietic Stem Cell Transplantation; Humans; Liver Cirrhosis - pathology; Liver Cirrhosis - therapy; Major thalassemia; Male; Transient elastography; Hematopoietic stem cell transplantation; Transient elastography; Major thalassemia
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2014.07.025

Abstract Although liver biopsy is an invasive procedure, it remains the gold standard technique for the evaluation of hepatic fibrosis in different patients, including those with major thalassemia (MT). Recently, noninvasive imaging techniques, such as transient elastography, have emerged. We investigated the effectiveness of TE, in comparison to liver biopsy, for the evaluation of liver fibrosis in pediatric patients with MT who were candidates for hematopoietic stem cell transplantation (HSCT). Eighty-three pediatric MT patients (48 boys and 35 girls), who were candidates for HSCT, were included in this study. The median age was 8 years. Liver stiffness was assessed for all patients, before transplantation, using both TE, measured in kilopascals (kPa) and liver biopsy, based on the Metavir score. The diagnostic accuracy of TE and liver biopsy were estimated using linear discriminated analysis (the area under the receiver operating characteristic curves [AUROCs]). The median TE score was 4.3 kPa (range, 3.5 to 5.2). The TE value did not differ among patients with different ferritin levels ( P  = .53). TE increased proportionally to Metavir fibrosis stages ( P < .001) and the necro-inflammatory grade ( P  < .001). The TE score also correlated to liver iron content ( P < .001), liver size ( P  < .003), and Lucarelli risk classification (LRC) ( P < .001). ROC curve analysis revealed moderate accuracy of the TE score for the diagnosis of fibrosis (AUROC = 73%) and for distinguishing individuals with a LRC III from those classified as I and II (AUROC = 82%). The TE score was also superior to Fibrosis-4 (AUROC = 61%) for the assessment of liver fibrosis and LRC differentiation. The results of this study demonstrated that TE can be a valuable method for assessing liver fibrosis and differentiating LRC III from the other 2 classes in pediatric patients with MT who have been selected for HSCT.