Circadian rhythm of RNA N6-methyladenosine and the role of cryptochrome
Methylation of RNA N6-methyladenosine has fundamental cellular functions, including translation regulation, RNA export, and stem cells renewal. However, the regulation of RNA N6-methyladenosine methylation is poorly understood. Here, we observed a robust circadian rhythm in N6-methyladenosine modifi...
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Published in | Biochemical and biophysical research communications Vol. 465; no. 1; pp. 88 - 94 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
11.09.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Methylation of RNA N6-methyladenosine has fundamental cellular functions, including translation regulation, RNA export, and stem cells renewal. However, the regulation of RNA N6-methyladenosine methylation is poorly understood. Here, we observed a robust circadian rhythm in N6-methyladenosine modifications of RNA. Deficiency of core mammalian clock genes, cryptochromes, decreased the levels of N6-methyladenosine in RNA. Cryptochrome1/2 knockout mice had significantly lower N6-methyladenosine methylation of RNA and lost the circadian rhythm of N6-methyladenosine levels in RNA. Global analysis of the circadian methylomes of N6-methyladenosine in RNA revealed that gene transcription, translation regulation, and RNA metabolism were highly correlated with N6-methyladenosine oscillation. Our findings extended a fundamental link between the circadian rhythm and N6-methyladenosine modification of RNA and suggested that this link is critical in controlling post-transcriptional gene expression and RNA metabolism.
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•N6-methyladenosine (m6A) modifications of RNA exhibit a robust circadian rhythm.•Deficiency of CRY decreases the levels of m6A modifications in RNA.•CRY1/2 knockout mice lose the circadian rhythm of m6A modifications in RNA.•Circadian m6A rhythms correlate with RNA metabolism and transcription/translation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2015.07.135 |