A SIMPLE SYSTEM FOR STUDYING ANTIBIOTIC BINDING TO LEUKOCYTES

Studies on the interaction between antibiotics and polymorphonuclear leukocytes (PMLs) usually require the availability of radiolabeled antibiotic, that the antibiotic kill intraleukocyte pathogens, or that the antibiotic affect some function of the leukocyte. The system described here does not have...

Full description

Saved in:
Bibliographic Details
Published inJournal of antibiotics Vol. 37; no. 3; pp. 267 - 274
Main Authors GRAY, GARY D., OHLMANN, GRETA M., FORD, CHARLES W., MARSHALL, VINCENT P.
Format Journal Article
LanguageEnglish
Published Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 01.01.1984
Japan Antibiotics Research Association
Subjects
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Centrifugation
Leukocytes - metabolism
Medical sciences
Neutrophils - metabolism
Phagocytosis
Pharmacology. Drug treatments
Protein Binding
Staphylococcus aureus - drug effects
Temperature
Antibiotic
Sensitivity
Investigation method
Leukocyte
Interaction
Bacteria
Mechanism of action
In vitro
Labelled compound
Staphylococcus aureus
Online AccessGet full text

Cover

More Information
Summary:Studies on the interaction between antibiotics and polymorphonuclear leukocytes (PMLs) usually require the availability of radiolabeled antibiotic, that the antibiotic kill intraleukocyte pathogens, or that the antibiotic affect some function of the leukocyte. The system described here does not have the requirements above but depends instead upon anti-staphylococci activity. The key features of the system are that, following incubation, extracellular antibiotic was removed from PMLs by centrifugation, contact between Staphylococcus aureus 502A and the phagocyte-antibiotic complex was assured by centrifugation at 4°C in 96 well tissue culture dishes, phagocytosis was induced by incubation at 37°C, and assessment of surviving bacteria was accomplished by collecting [3H]thymidine labeled bacteria using a cell harvester. Antibiotics that were active in this system included naphthalenic ansamycins (rifamycins and streptovaricins), lincosaminides (clindamycins and pirlimycins), coumarins (novobiocin), erythromycin, tetracycline, tyrocidine and paulomycin.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.37.267