Efficient Agrobacterium-mediated Transformation of The Elite–Indica Rice Variety Komboka

Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice ( L.) is an important model in plant research, since it is the staple food for more than half of the world's population. As a result, numerous transformation method...

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Published inBio-protocol Vol. 10; no. 17; p. e3739
Main Authors Luu, Van, Stiebner, Melissa, Maldonado, Paula, Valdés, Sandra, Marín, Didier, Delgado, Gerardo, Laluz, Virginia, Wu, Lin-Bo, Chavarriaga, Paul, Tohme, Joe, Slamet-Loedin, Inez, Frommer, Wolf
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.09.2020
Bio-protocol LLC
Subjects
Biology
Methods
Rice transformation
Oryza sativa
Elite variety
Indica rice
Agrobacterium-mediated transformation
Plant regeneration
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Summary:Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice ( L.) is an important model in plant research, since it is the staple food for more than half of the world's population. As a result, numerous transformation methods have been developed for both and rice. Since breeders continuously develop new rice varieties, transformation protocols have to be adapted for each new variety. Here we provide an optimized transformation protocol with detailed tips and tricks for a new African variety Komboka using immature embryos. In Komboka, we obtained an apparent transformation rate of up to 48% for GUS/GFP reporter gene constructs using this optimized protocol. This protocol is also applicable for use with other elite rice varieties.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3739