Globular adiponectin regulates energy homeostasis through AMP-activated protein kinase-acetyl-CoA carboxylase (AMPK/ACC) pathway in the hypothalamus

• Published in: Molecular and cellular biochemistry Vol. 344; no. 1-2; pp. 109 - 115
• Main Authors: Wen, Jun-Ping, Liu, Chun-e, Hu, Ya-Ting, Chen, Gang, Lin, Li-xiang
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.11.2010; Springer US; Springer; Springer Nature B.V
• Subjects: ACC; Acetyl-CoA Carboxylase; Acetyl-CoA Carboxylase - metabolism; adiponectin; Adiponectin - physiology; AgRP; AMP-Activated Protein Kinases; AMP-Activated Protein Kinases - metabolism; AMPK; Animals; Base Sequence; Biochemistry; Biomedical and Life Sciences; Blotting, Western; Cardiology; Cell Line; Cellular biology; DNA Primers; Energy; Energy homeostasis; Energy Metabolism; enzymology; Homeostasis; Hypothalamus; Hypothalamus - enzymology; Hypothalamus - metabolism; Kinases; Life Sciences; Medical Biochemistry; metabolism; Mice; Molecular biology; Oncology; Peptides; Phosphorylation; physiology; Protein kinases; Reverse Transcriptase Polymerase Chain Reaction; RNA; Rodents; Signal transduction; STAT3 Transcription Factor; STAT3 Transcription Factor - metabolism; ACC; AMPK; Energy homeostasis; AgRP; Adiponectin
• ISSN: 0300-8177; 1573-4919; 1573-4919
• DOI: 10.1007/s11010-010-0534-2

Adiponectin is a newly researched adipokine which participates in the regulation of energy homeostasis. AMP-activated protein kinase (AMPK) represents an energy sensor that responds to hormone and nutrition status in vivo and exerts a regulatory effect in the hypothalamus and multiple peripheral tissues. We investigated the possible mechanisms involved in appetite regulation by adiponectin in vitro with GT1-7 cells, a mouse immortalized hypothalamic neuron. The results showed that adiponectin increased the phosphorylation of AMPK, activated AMPK phosphorylated and inactivated acetyl-CoA carboxylase (ACC), and subsequently increased expression of agouti-related peptide (AgRP) mRNA. Our results also indicated that adiponectin had no effect on signal transducer and activator of transcription (STAT3). Together these findings suggest that adiponectin regulated energy homeostasis through the AMPK/ACC pathway but not the JAK/STAT3 pathway in the hypothalamus.