Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature

Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effe...

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Published inPolymers Vol. 12; no. 1; p. 213
Main Authors Kojima, Chie, Nishio, Misaki, Nakajima, Yusuke, Kawano, Takeshi, Takatsuka, Kenji, Matsumoto, Akikazu
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.01.2020
MDPI
Subjects
Biomedical materials
cell detachment
Collagen
denaturation
Fourier transforms
Gels
Gold
gold nanoparticle
Heating
Hydrogels
Infrared analysis
Lasers
Nanoparticles
photoinduced
Rheological properties
Rheology
Shear strain
Sol-gel processes
Spectrum analysis
telopeptide
Thermal denaturation
Japan
collagen
telopeptide
denaturation
photoinduced
cell detachment
gold nanoparticle
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Summary:Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effect of AuNP. Physicochemical properties of collagen materials depend on the origin of the collagen and the presence of telopeptides. In this study, we prepared various AuNP-embedded collagen gels by using different collagen materials with and without the telopeptides to compare their thermal denaturation properties and photoinduced single cell detachment behaviors. Cellmatrix type I-C without telopeptides exhibited a lower denaturation temperature than Cellmatrix type I-A and Atelocell IAC, as examined by Fourier transform infrared (FTIR) spectroscopy, rheological analysis, and sol-gel transition observation. Three-dimensional (3D) laser microscopic imaging revealed that collagen fibers shrank in Cellmatrix type I-A upon heating, but collagen fibers disappeared in Cellmatrix type I-C upon heating. Cells cultured on the Cellmatrix type I-C-based AuNP-embedded collagen gel detached with shorter photoirradiation than on the Cellmatrix type I-A-based AuNP-embedded collagen gel, suggesting that collagen gels without telopeptides are suitable for a photoinduced single cell detachment system.
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ISSN:2073-4360
2073-4360
DOI:10.3390/polym12010213