Pinostrobin: An Adipogenic Suppressor from Fingerroot (Boesenbergia rotunda) and Its Possible Mechanisms

• Published in: Foods Vol. 11; no. 19; p. 3024
• Main Authors: San, Htoo Tint, Khine, Hnin Ei Ei, Sritularak, Boonchoo, Prompetchara, Eakachai, Chaotham, Chatchai, Che, Chun-Tao, Likhitwitayawuid, Kittisak
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 29.09.2022; MDPI
• Subjects: Adipocytes; Adipogenesis; Akt; AKT protein; Apoptosis; Body fat; Body mass index; Boesenbergia rotunda; Cell cycle; Coronaviruses; COVID-19; Differentiation; Fecal incontinence; fingerroot; Flavonoids; Food science; Gastrointestinal surgery; Insulin; Kinases; Lipid metabolism; Lipids; MAP kinase; MAPK; Metabolic disorders; Metabolic syndrome; Obesity; Peroxisome proliferator-activated receptors; Proteins; Sterol regulatory element-binding protein; Triglycerides; Weight control; Thailand; United States > US; Indonesia; India
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods11193024

Obesity is a critical factor for chronic metabolic syndromes. The culinary plant fingerroot (Boesenbergia rotunda) has been reported for its anti-obesity activity. The anti-adipogenic effects of pandurantin A, a main component of fingerroot cultivated in Indonesia, have been studied. Nevertheless, the suppressive effect and related mechanisms of pinostrobin, a major constituent of Thai fingerroot, on adipogenesis have never been thoroughly investigated. This study aimed to evaluate the potential of pinostrobin to inhibit adipocyte differentiation. Culturing pre-adipocytes from both mouse (3T3-L1) and human (PCS-210-010) with pinostrobin at non-toxic concentrations (5−20 µM) for 48 h obviously hindered their differentiation into mature adipocyte as evidenced by reduced cellular lipid droplets. The lower levels of lipid metabolism-mediating proteins, namely C/EBPα, PPARγ, and SREBP-1c, as well as cellular triglyceride content were demonstrated in pinostrobin-treated 3T3-L1 cells when compared to the untreated control group. Additionally, pinostrobin modulated the signals of MAPK (p38 and JNK) and Akt (Akt/GSK3β, Akt/AMPKα-ACC). These findings suggest the benefit of fingerroot as a source of phytopharmaceuticals for obesity prevention and management, with pinostrobin as the active principle.