Sensing and antibacterial activity of imidazolium-based conjugated polydiacetylenes

• Published in: Biosensors & bioelectronics Vol. 77; pp. 1016 - 1019
• Main Authors: Lee, Songyi, Cheng, Hua, Chi, Meiying, Xu, Qingling, Chen, Xiaoqiang, Eom, Chi-Yong, James, Tony D., Park, Sungsu, Yoon, Juyoung
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2016
• Subjects: Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemical synthesis; Antibacterial effect; Antiinfectives and antibacterials; Bacteria; Biosensors; Cell Line; Cell Survival - drug effects; Charging; Colorimetric sensor; Colorimetry - instrumentation; Detection; Equipment Design; Equipment Failure Analysis; Escherichia coli; Fluorescent sensor; Humans; Imidazoles - administration & dosage; Imidazoles - chemistry; Materials Testing; PDA; Polyacetylenes - administration & dosage; Polyacetylenes - chemical synthesis; Polydiacetylenes; Polymers - administration & dosage; Polymers - chemical synthesis; Probe-killer; Reproducibility of Results; Salmonella typhimurium - drug effects; Salmonella typhimurium - isolation & purification; Salmonella typhimurium - physiology; Sensitivity and Specificity; Staphylococcus aureus; Fluorescent sensor; Polydiacetylenes; Colorimetric sensor; Probe-killer; Antibacterial effect
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.10.090

In the current study, we report the first example of polydiacetylenes (PDAs), where our PDA-based system acts as both a sensing probe and killer for bacteria. The contact of imidazolium and imidazole-derived PDA with various bacterial strains including MRSA (methicillin-resistant Staphylococcus aureus) and ESBL-EC (extended-spectrum β-lactamase-producing Escherichia coli) results in a distinct blue-to-red colorimetric change of the solution as well as a rapid disruption of the bacterial membrane, which is demonstrated by transmission electron microscopy and confocal microscopy. Zeta potential analysis supports that antibacterial activity of the PDA solution originates from an electrostatic interaction between the negatively charged bacterial cell surface and the positively charged polymers. These results suggest that the PDA has a great potential to carry out the dual roles of a probe and killer for bacteria. [Display omitted] •The new PDAs showed colorimetric change, with bacterial growth inhibition.•Evidence was gained involves Imidazolium of PDAs by TEM and confocal microscopy.•Bacterial invasion into the human intestinal epithelial cells by S.typhimurium was reduced by the PDAs.