Production and characterization of ectoine using a moderately halophilic strain Halomonas salina BCRC17875

• Published in: Journal of bioscience and bioengineering Vol. 125; no. 5; pp. 578 - 584
• Main Authors: Chen, Wei-Chuan, Hsu, Ching-Cha, Lan, John Chi-Wei, Chang, Yu-Kaung, Wang, Li-Fen, Wei, Yu-Hong
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.05.2018
• Subjects: agitation; ammonium sulfate; carbon; carbon nitrogen ratio; chemical structure; commercialization; Ectoine; fermentation; Halomonas salina; Identification; mass spectrometry; Medium optimization; nitrogen; nitrogen content; nuclear magnetic resonance spectroscopy; sodium chloride; yeast extract; Identification; Ectoine; Medium optimization; Halomonas salina
• ISSN: 1389-1723; 1347-4421; 1347-4421
• DOI: 10.1016/j.jbiosc.2017.12.011

This study attempted to utilize Halomonas salina BCRC17875 to produce ectoine by optimizing the agitation speed and medium composition. In addition, the chemical structure of ectoine produced by H. salina BCRC17875 was determined. The results indicate that ectoine production reached 3.65 g/L at 38 h of cultivation when the agitation rate and NaCl concentration were fixed at 200 rpm and 2.0 M, respectively. It reached 9.20 g/L at 44 h of cultivation when the major medium components were yeast extract (56 g/L), glutamate (74.40 g/L), and ammonium sulfate (14 g/L). After the nitrogen concentration had been evaluated, evaluation of the nitrogen concentration revealed that the ectoine production reached 11.80 g/L at 44 h of cultivation when 56 g/L of yeast extract and 28 g/L of ammonium sulfate were used. Ectoine production reached 13.96 g/L at 44 h of cultivation when the carbon/nitrogen ratio was fixed at 3/1 using 84 g/L of yeast extract and 28 g/L of ammonium sulfate. Furthermore, the identification of ectoine were identified and characterized by fast atom bombardment mass spectrometry (FAB-MS) and 1H NMR. The results demonstrated a fermentation strategy was successful in increasing ectoine production, and that the fermentation medium of ectoine had commercialization potential. •Structure of ectoine from the culture of Halomonas salina BCRC17875 was identified and characterized by FAB-MS and 1H NMR.•Effects of agitation rate and sodium chloride concentration on ectoine production were studied.•Effects of the types of carbon and nitrogen sources on ectoine production were determined.•High ectoine production (13.96 g/L) achieved when a carbon/nitrogen ratio of 3/1 was utilized.