Anthocyanin supplementation improves anti-oxidative and anti-inflammatory capacity in a dose–response manner in subjects with dyslipidemia

• Published in: Redox biology Vol. 32; p. 101474
• Main Authors: Zhang, Hanyue, Xu, Zhongliang, Zhao, Huiwen, Wang, Xu, Pang, Juan, Li, Qing, Yang, Yan, Ling, Wenhua
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2020; Elsevier
• Subjects: 8-Hydroxy-2′-deoxyguanosine; 8-iso-prostaglandinF2α; Anthocyanins; Interleukin-6; Randomized controlled trial; Research Paper; CRP; LDL-C; BH; 8-iso-PGF2; FBG; MDA; UA; WC; BP; Interleukin-6; HDL-C; IFN-γ; 8-Hydroxy-2′-deoxyguanosine; DBP; BW; 8-iso-prostaglandinF2α; T-SOD; 8-OHdG; BMI; WHR; SBP; FINS; IL-1β; Randomized controlled trial; IL-6; TC; HOMA-IR; VCAM-1; TG; NC; ROS; Anthocyanins; HC; CHD; 8-iso-prostaglandinF(α)
• ISSN: 2213-2317; 2213-2317
• DOI: 10.1016/j.redox.2020.101474

Anthocyanins, one of the major plant bioactive substances, possess anti-oxidative and anti-inflammatory capacity. However, their dose–response relationship has remained unclear. The present study investigated the dose–response relationship of anthocyanins with oxidative stress and inflammation in subjects with dyslipidemia. and Participants: A total of 169 participants with dyslipidemia were randomly assigned to placebo (n = 43), anthocyanins 40 mg/day (n = 44), 80 mg/day (n = 40), or 320 mg/day (n = 42) groups. Urine 8-iso-prostaglandin F2α (8-iso-PGF2α), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and serum malonaldehyde (MDA), total superoxide dismutase (T-SOD), UA (uric acid), interleukin (IL)-6, IL-10, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) were measured at baseline, at 6 weeks, and at 12 weeks. Anthocyanin supplementation (320 mg/day) for 6 weeks significantly improved T-SOD versus baseline (P < 0.05). A slight reduction in serum IL-6, TNF-α, and urine 8-iso-PGF2α from the baseline was observed at 12 weeks in the group receiving 40 mg/day anthocyanins. Anthocyanins (80 mg/day) significantly reduced serum IL-6 (−20%), TNF-α (−11%) and urine 8-iso-PGF2α (−27%) versus baseline (P < 0.05). Moreover, 320 mg/day anthocyanin supplementation reduced serum IL-6 (−40%), TNF-α (−21%), MDA (−20%) and urine 8-iso-PGF2α (−37%) and 8-OHdG (−36%) than 80 mg/day and 40 mg/day anthocyanins, P value < 0.05. Anthocyanin supplementation has dose-response relationships with decreased inflammatory cytokines IL-6, TNF-α and oxidative stress biomarkers 8-iso-PGF2α, 8-OHdG and MDA (P for trend, <0.05). Furthermore, a strong positive correlation was observed between the changes in the urine 8-iso-PGF2α , 8-OHdG levels and serum IL-6 levels in subjects from anthocyanin groups after 12 weeks of treatment. Supplementation of anthocyanins for 12 weeks positively improved the anti-oxidative and anti-inflammatory capacity in a dose–response manner in individuals with dyslipidemia. [Display omitted] •Anthocyanins improve oxidative stress in a dose-response manner.•Anthocyanins improve anti-inflammatory capacity in a dose-response manner.•80 mg/day anthocyanin could reach the threshold level for producing beneficial functions after 12-week intervention.•Anthocyanins prevent the progression of metabolic diseases in subjects with dyslipidemia.