CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber

Flavonols and proanthocyanidins (PAs) are the main pigments in the black spines of cucumber ( ) fruit, and is a key regulator of the biosynthesis of flavonols and PAs. However, in cucumber, the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by rema...

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Bibliographic Details
Published inHorticulture research Vol. 7; no. 1; p. 103
Main Authors Li, Jialin, Luan, Qianqian, Han, Jing, Zhang, Cunjia, Liu, Mengyu, Ren, Zhonghai
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.07.2020
Nature Publishing Group UK
Subjects
Biosynthesis
Breeding
Flavonoids
Flavonols
Fruits
Gene regulation
Pigments
Proanthocyanidins
Proteins
Transcription
Molecular biology
Genetics
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Summary:Flavonols and proanthocyanidins (PAs) are the main pigments in the black spines of cucumber ( ) fruit, and is a key regulator of the biosynthesis of flavonols and PAs. However, in cucumber, the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by remain unclear. In this study, we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber. CsMYB60 activated and by binding to their promoters and directly or indirectly promoted the expression of , , , and , resulting in the formation of complexes of these four proteins to increase the expression of and interact with CsTATA-box binding protein to regulate the expression of , thereby regulating the biosynthesis of flavonols and PAs in cucumber. Our data provide new insights into the molecular mechanism of flavonoid biosynthesis, which will facilitate molecular breeding to improve fruit quality in cucumber.
ISSN:2662-6810
2052-7276
DOI:10.1038/s41438-020-0327-z