Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells

• Published in: Neuron (Cambridge, Mass.) Vol. 90; no. 5; pp. 1043 - 1056
• Main Authors: Szoboszlay, Miklos, Lőrincz, Andrea, Lanore, Frederic, Vervaeke, Koen, Silver, R. Angus, Nusser, Zoltan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2016; Elsevier Limited; Elsevier; Cell Press
• Subjects: Animals; cerebellum; Cerebellum - cytology; connexin36; Connexins - metabolism; dendrites; Dendrites - physiology; electrical synapses; Electrical Synapses - physiology; Female; Gap Junction delta-2 Protein; gap junctions; Gap Junctions - physiology; Immunoglobulins; immunolocalization; interneuron; Interneurons - physiology; Life Sciences; Male; Mice; Neurons and Cognition; Rodents; Studies; connexin36; gap junctions; immunolocalization; dendrites; interneuron; cerebellum; electrical synapses
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2016.03.029

The strength and variability of electrical synaptic connections between GABAergic interneurons are key determinants of spike synchrony within neuronal networks. However, little is known about how electrical coupling strength is determined due to the inaccessibility of gap junctions on the dendritic tree. We investigated the properties of gap junctions in cerebellar interneurons by combining paired somato-somatic and somato-dendritic recordings, anatomical reconstructions, immunohistochemistry, electron microscopy, and modeling. By fitting detailed compartmental models of Golgi cells to their somato-dendritic voltage responses, we determined their passive electrical properties and the mean gap junction conductance (0.9 nS). Connexin36 immunofluorescence and freeze-fracture replica immunogold labeling revealed a large variability in gap junction size and that only 18% of the 340 channels are open in each plaque. Our results establish that the number of gap junctions per connection is the main determinant of both the strength and variability in electrical coupling between Golgi cells. •The mean conductance of dendritic gap junctions between Golgi cells is 0.9 nS•Dendritic gap junctions have 340 connexin36 channels of which 18% are open•Dendritic location contributes little to coupling strength variability•Gap junction number is the main determinant of coupling strength variability Variation in the strength of electrical synapses influences spike synchrony in interneuron networks. Szoboszlay et al. investigated the properties of electrical synapses between cerebellar Golgi cells and showed that the number of gap junctions is the main determinant of coupling strength variability.