High-Affinity Reactions Between Antigen-Specific T-Cell Receptors and Peptides Associated with Allogeneic and Syngeneic Major Histocompatibility Complex Class I Proteins
We report here that the intrinsic affinities of the antigen-specific T-cell receptors (TCR) of two unrelated CD8+T-cell clones for their respective peptide-major histocompatibility complex (MHC) ligands are higher than the values generally thought to prevail for TCR. The TCR of one clone (2C) binds...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 24; pp. 11487 - 11491 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
22.11.1994
National Acad Sciences National Academy of Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | We report here that the intrinsic affinities of the antigen-specific T-cell receptors (TCR) of two unrelated CD8+T-cell clones for their respective peptide-major histocompatibility complex (MHC) ligands are higher than the values generally thought to prevail for TCR. The TCR of one clone (2C) binds an allogeneic class I MHC protein (Ld) in association with an α-ketoglutarate dehydrogenase nonapeptide (QLSPFPFDL, termed QL9) with an intrinsic affinity (intrinsic equilibrium association constant) of 1-2 x 107M-1. The TCR of the other clone (4G3) binds a syngeneic class I MHC protein (Kb) in association with an ovalbumin octapeptide (SIINFEKL, termed pOV8) with an intrinsic affinity of 1.5 x 106M-1. A comparison of the two clones, combined with current views of T-cell repertoire selection in the thymus, leads us to propose that TCR affinities are generally likely to be higher for allogeneic MHC-peptide complexes than for syngeneic MHC-peptide complexes. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.91.24.11487 |