Immunological microenvironment gene expression in patients with diffuse large B cell non Hodgkin lymphoma

• Published in: Biochemistry and biophysics reports Vol. 21; p. 100731
• Main Authors: Elhelbawy, Nesreen G, Nassar, Adel A.H., Eltorgoman, Abd ElMonem A, Saber, Safa M, Badr, Eman AE
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2020; Elsevier
• Subjects: Diffuse large B cell non Hodgkin lymphoma (DLBCL); Gene expression; Real time PCR; Gene expression; Diffuse large B cell non Hodgkin lymphoma (DLBCL); Real time PCR
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2020.100731

Non Hodgkin lymphoma (NHL) is one of the immune system cancers. The occurrence and progression of malignant lymphomas depends on cellular pathways deregulation. Understanding the relationship between the immune system at the genetic level and malignant transformation is critical to reach its etiology. The aim of this work is to evaluate the expression of five immune related genes (PD-1, FOXP3, GrA, GrB and CD11c) in patients with diffuse large B cell non Hodgkin lymphoma (DLBCL). This study was conducted on fifty patients with DLBCL and fifty sex and age matched apparently healthy subjects. The participants were subjected to these laboratory investigations: complete blood count, serum lactate dehydrogenase and β2microglobulin (β2M) levels and determination of PD-1, FOXP3, GrA, GrB and CD11c gene expressions. The results of this study revealed that PD-1, FOXP3, GrA, GrB and CD11c gene expressions were significantly increased in DLBCL patients. Patients with DLBCL have variablePD-1, FOXP3,GrA, GrB and CD11cgene expressions levels, which are correlated with the overall survival (OS) indicating that they can be good predictors of outcome in these patients. •The gene expression of immunity related genes are important indicators of lymphoma overall survival and prognosis.•Patients with DLBCL is usually accompanied by increased PD-1, and FOXP3 gene expression.•GrA and GrB genes expression decrease in DLBCL.•CD11c gene expression level is variable.