Upregulation of S100 calcium-binding protein A9 is required for induction of smooth muscle cell proliferation by a periodontal pathogen

• Published in: FEBS letters Vol. 583; no. 1; pp. 128 - 134
• Main Authors: Inaba, Hiroaki, Hokamura, Kazuya, Nakano, Kazuhiko, Nomura, Ryota, Katayama, Kazufumi, Nakajima, Atsushi, Yoshioka, Hideo, Taniguchi, Kazuhiro, Kamisaki, Yoshinori, Ooshima, Takashi, Umemura, Kazuo, Murad, Ferid, Wada, Koichiro, Amano, Atsuo
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.01.2009
• Subjects: Aorta - metabolism; Aorta - microbiology; Aorta - pathology; Calgranulin B - genetics; Calgranulin B - metabolism; Cell Proliferation; Cells, Cultured; embryonic isoform of myosin heavy chain; Gene Knockdown Techniques; hAOSMC; Human; human aortic smooth muscle cell; Humans; Hyperplasia; Myocytes, Smooth Muscle - metabolism; Myocytes, Smooth Muscle - microbiology; Myocytes, Smooth Muscle - pathology; P. gingivalis; Periodontium - microbiology; Porphyromonas gingivalis; Proliferation; S100; siRNA; small interfering RNA; SMC; SMemb; Smooth muscle cell; Tunica Intima - metabolism; Tunica Intima - microbiology; Tunica Intima - pathology; Up-Regulation; Smooth muscle cell; Human; Porphyromonas gingivalis; P. gingivalis; hAOSMC; SMemb; SMC; S100; siRNA; Proliferation
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2008.11.036

We investigated the effect of a periodontal pathogen, Porphyromonas gingivalis, on human aortic smooth muscle cell (hAOSMC) proliferation as mechanisms of atherosclerosis. Cultured hAOSMCs exposed to the supernatant of plasma incubated with P. gingivalis showed a marked transformation from a contractile to proliferative phenotype, resulting in enhancement of cell growth. DNA microarray analysis revealed a P. gingivalis-dependent upregulation of S100A9 in hAOSMCs. Small interference-RNA for S100A9 dramatically attenuated the effect of P. gingivalis on transformation and proliferation of hAOSMCs. Our data suggested that upregulation of S100A9 mediated by P. gingivalis is an important event in the development of aortic intimal hyperplasia.