The deformation matrix theory of basement membrane: a study of water flow through elastic and rigid filaments in the rat
1. When the volume of water per unit time which flows through natural elastic basement membrane is divided by the applied pressure, the value-the hydraulic conductivity-is not constant but decreases as pressure increases. In contrast when the same membrane is tanned with glutaraldehyde and rendered...
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Published in | The Journal of physiology Vol. 406; no. 1; pp. 1 - 14 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
Oxford
The Physiological Society
01.12.1988
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | 1. When the volume of water per unit time which flows through natural elastic basement membrane is divided by the applied
pressure, the value-the hydraulic conductivity-is not constant but decreases as pressure increases. In contrast when the same
membrane is tanned with glutaraldehyde and rendered inelastic, the hydraulic conductivity is constant at all pressures. 2.
Over a pressure range of 0-6.7 kPa equivalent to a membrane stress of 0-195 kPa in natural elastic membrane the hydraulic
conductivity (Lp) can be related by the linear equation Lp = Lp.0 + apP where P is the hydraulic pressure, Lp.0 is the initial
hydraulic conductivity and ap is a constant which is the decreased hydraulic conductivity per unit pressure (correlation coefficient
0.764. P less than 0.001). 3. The initial conductivity of the basement membrane of the crystalline lens of the adult rat (lens
capsule) was 47.6 +/- 7.3 x 10(-12) m s-1 Pa-1 while the decrease in hydraulic conductivity per unit increase in pressure
was -3.38 x 10(-15) m s-1 Pa-2. 4. Following tanning with glutaraldehyde the hydraulic conductivity was constant at 27.4 +/-
4.0 x 10(-12) m s-1 Pa-1. 5. A change in the configuration of the superhelices of the filaments of type IV collagen which
form the framework of basement membrane is termed. 'The deformation matrix theory' and can satisfactorily account for the
changes in hydraulic conductivity of both natural and tanned membrane. 6. In natural membrane the filaments deform easily
and the pitch of the filament superhelices is increased by axial stress induced by pressure. The filaments straighten and
become compacted together and the hydraulic permeability is thereby decreased. 7. In tanned membrane the filaments become
more rigid and axial stress barely deforms them: moreover the pitch of the filament superhelices is decreased so that the
filaments become more closely coiled and compacted together. Because of these changes the hydraulic conductivity is reduced
as compared with unstressed natural membrane and remains unaltered by increasing pressure. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.1988.sp017364 |