Cloning and expression analysis of YY1AP-related protein in the rat brain

• Published in: Amino acids Vol. 34; no. 1; pp. 155 - 161
• Main Authors: Ohtomo, T, Horii, T, Nomizu, M, Suga, T, Yamada, J
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer-Verlag 01.01.2008; Springer-Verlag; Springer Nature B.V
• Subjects: Analytical Chemistry; Animals; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Brain; Brain - metabolism; Cloning, Molecular; dingoes; DNA, Complementary - genetics; Gene Expression Regulation; Genome - genetics; GON4L; In Situ Hybridization; Life Sciences; Male; Neurobiology; Neuron; Organ Specificity; Proteins; Proteomics; Rats; RNA, Messenger - genetics; Rodents; Testis - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic - genetics; Transcriptional coactivator; YARP; YY1-associated protein; Keywords: YY1-associated protein – YARP – Dingo – GON4L – Transcriptional coactivator – Neuron
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-006-0483-y

YY1AP-related protein (YARP) is a structural homolog of YY1AP, a transcriptional coactivator of the multifunctional transcription factor YY1. We cloned a rat YARP cDNA that encoded a 2256 amino acid protein with 93% homology to the human counterpart. Northern blots revealed significant expression of the YARP gene in the rat brain. In situ hybridization demonstrated its expression in neurons throughout the brain, including pyramidal cells in the cerebral cortex and hippocampus and granule cells in the dentate gyrus. YARP was coexpressed with YY1 in these same neuronal cells. However, there was no evidence of YARP expression in glia. In the developing rat brain, the level of YARP mRNA (~10 kb) peaked at embryonic day 18 and promptly declined thereafter to reach the steady-state level found in adulthood, by 14 days after birth. These results suggest that YARP functions at a late stage of neurogenesis during perinatal development of the rat brain, as well as in mature neurons.