Promoter region polymorphisms in the transforming growth factor beta-1 (TGFβ1) gene and serum TGFβ1 concentration in preeclamptic and control Iranian women

• Published in: Journal of reproductive immunology Vol. 94; no. 2; pp. 216 - 221
• Main Authors: Feizollahzadeh, Sadegh, Taheripanah, Robabeh, Khani, Masood, Farokhi, Babak, Amani, Dawar
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.06.2012
• Subjects: Adolescent; Adult; Female; Gene Frequency; Genetic Association Studies; Genotype; Humans; Iran; Mutation - genetics; Obstetrics and Gynecology; Polymorphism; Polymorphism, Genetic; Pre-eclampsia; Pre-Eclampsia - genetics; Pre-Eclampsia - immunology; Pregnancy; Promoter Regions, Genetic - genetics; TGFβ1; Transforming Growth Factor beta1 - biosynthesis; Transforming Growth Factor beta1 - blood; Transforming Growth Factor beta1 - genetics; Young Adult; Iran; RSA; TGFβ1; Iran; PE; Pre-eclampsia; RFLP; Polymorphism; restriction fragment length polymorphism; recurrent spontaneous abortion; pre-eclampsia
• ISSN: 0165-0378; 1872-7603; 1872-7603
• DOI: 10.1016/j.jri.2012.02.006

Preeclampsia (PE) is a pregnancy associated disorder characterized by hypertension and proteinuria, which causes neonatal and maternal morbidity and mortality. The Th1/Th2 cytokine paradigm of the immune adaptation in pregnancy is now expanded to include Th1/Th2/Th17 and regulatory T (Treg) cells. Among cytokines, TGFβ1 has properties that justify evaluation of its role in PE etiopathology. In this investigation the polymorphisms of the TGFβ1 gene at promoter region, positions −800G→A and −509C→T, were studied in 142 PE and 140 normal pregnant female subjects using PCR-RFLP. Additionally, serum TGFβ1 was determined by ELISA. At position −800G→A genotypes and allele frequencies showed no significant differences between PE patients (GG 73.9%; GA 21.1%; AA 4.93%) and normal control (GG 70%; GA 28.6%; AA 1.4%) women. However the AA genotype at this position was more frequent in PE patients than in the control group. At −509C→T position, genotypes and allele frequencies showed no significant differences between PE patients and control individuals. The CC genotype at −509C→T position was more prevalent in PE patients than in the control group. The mean serum TGFβ1 level was significantly higher (62.14ng/ml) in PE patients compared with pregnant and non-pregnant control groups (and 47.01 and 40.68ng/ml, respectively). In conclusion, the promoter region polymorphisms of TGFβ1 may not be associated with PE, but serum levels of this cytokine may contribute to the etiopathology of PE.