Inhibitory Effect of the Essential Oil from Chamaecyparis obtusa on the Growth of Food-Borne Pathogens

• Published in: The journal of microbiology Vol. 48; no. 4; pp. 496 - 501
• Main Authors: Park, M.J., Korea Forest Research Institute, Seoul, Republic of Korea, Choi, W.S., Korea Forest Research Institute, Seoul, Republic of Korea, Kang, H.Y., Korea Forest Research Institute, Seoul, Republic of Korea, Gwak, K.S., Seoul National University, Seoul, Republic of Korea, Lee, G.S., Chungbuk National University, Cheongju, Republic of Korea, Jeung, E.B., Chungbuk National University, Cheongju, Republic of Korea, Choi, I.G., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.08.2010; Springer Nature B.V; 한국미생물학회
• Subjects: ACEITES ESENCIALES; Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - pharmacology; antibacterial activity; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Biomedical and Life Sciences; Chamaecyparis - chemistry; CHAMAECYPARIS OBTUSA; E coli; Escherichia coli; ESSENTIAL OILS; Female; Food contamination & poisoning; Food Microbiology; Foodborne Diseases - drug therapy; Foodborne Diseases - microbiology; Fungi; Growth Inhibitors - pharmacology; HUILE ESSENTIELLE; Humans; Klebsiella pneumoniae; Legionella pneumophila; Life Sciences; Listeria; Listeria monocytogenes; Mice; Mice, Inbred ICR; Microbiology; Microorganisms; Oils & fats; Oils, Volatile - analysis; Oils, Volatile - pharmacology; Pathogens; Plant Oils - analysis; Plant Oils - pharmacology; Research centers; Salmonella; Salmonella typhimurium; Staphylococcus aureus; Staphylococcus infections; Terpenes - analysis; Terpenes - pharmacology; terpinen-4-ol; 생물학; South Korea; Japan; antibacterial activity; terpinen-4-ol; essential oil
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-010-9327-2

In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed. C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus.