Effects of in vitro maturation on gene expression in rhesus monkey oocytes

• Published in: Physiological genomics Vol. 35; no. 2; pp. 145 - 158
• Main Authors: Lee, Young S, Latham, Keith E, VandeVoort, Catherine A
• Format: Journal Article
• Language: English
• Published: United States Am Physiological Soc 08.10.2008; American Physiological Society
• Subjects: 3' Untranslated Regions; Animals; Binding Sites; Call for Papers: Comparative Genomics; Female; Gene Expression; Gene Regulatory Networks; Genomic Imprinting; In Vitro Techniques; Macaca mulatta; Metaphase; Oocytes - growth & development; Oocytes - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 1094-8341; 1531-2267
• DOI: 10.1152/physiolgenomics.90281.2008

1 Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 2 Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 3 California National Primate Research Center, University of California, Davis, California 4 Department of Obstetrics and Gynecology, University of California, Davis, California In vitro oocyte maturation (IVM) holds great promise as a tool for enhancing clinical treatment of infertility, enhancing availability of nonhuman primates for development of disease models, and facilitating endangered species preservation. However, IVM outcomes have remained significantly below the success rates obtained with in vivo matured (VVM) oocytes from humans and nonhuman primates. A cDNA array-based analysis is presented, comparing the transcriptomes of VVM oocytes with IVM oocytes. We observe a small set of just 59 mRNAs that are differentially expressed between the two cell types. These mRNAs are related to cellular homeostasis, cell-cell interactions including growth factor and hormone stimulation and cell adhesion, and other functions such as mRNA stability and translation. Additionally, we observe in IVM oocytes overexpression of PLAGL1 and MEST , two maternally imprinted genes, indicating a possible interruption or loss of correct epigenetic programming. These results indicate that, under certain IVM conditions, oocytes that are molecularly highly similar to VVM oocytes can be obtained; however, the interruption of normal oocyte-somatic cell interactions during the final hours of oocyte maturation may preclude the establishment of full developmental competence. assisted reproduction; nonhuman primate; microarray