Compounds from Wedelia chinensis synergistically suppress androgen activity and growth in prostate cancer cells

• Published in: Carcinogenesis (New York) Vol. 28; no. 12; pp. 2521 - 2529
• Main Authors: Lin, Feng-Min, Chen, Li-Ru, Lin, En-Hau, Ke, Ferng-Chun, Chen, Hsin-Yi, Tsai, Meng-Jen, Hsiao, Pei-Wen
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.12.2007; Oxford Publishing Limited (England)
• Subjects: Androgen Antagonists - pharmacology; Androgen Receptor Antagonists; Anticarcinogenic Agents - pharmacology; Antineoplastic Agents, Phytogenic - pharmacology; Apigenin - pharmacology; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Cell Line, Tumor; Cell Proliferation - drug effects; Coumarins - pharmacology; Drug Synergism; Gynecology. Andrology. Obstetrics; Humans; Indoles - pharmacology; Luteolin - pharmacology; Male; Male genital diseases; Medical sciences; Neoplasms, Hormone-Dependent; Plant Extracts - pharmacology; Prostate-Specific Antigen - metabolism; Prostatic Neoplasms; Receptors, Androgen - genetics; Tumors; Wedelia - chemistry; Urinary system disease; Prostate disease; Androgen; Growth; Malignant tumor; Sex steroid hormone; Compounds; Male genital diseases; Prostate cancer; Biological activity; Tumor cell; Cancer
• ISSN: 0143-3334; 1460-2180
• DOI: 10.1093/carcin/bgm137

Chronic inflammation can augment tumor development in various types of cancers, including prostate cancer (PCa). Reduction of inflammation is therefore an important anticancer therapeutic opportunity. Here, we report four anti-proliferative phytocompounds in Wedelia chinensis, an oriental herbal medicine, identified through their ability to modulate the androgen receptor (AR) activation of transcription from prostate-specific antigen promoter in PCa cells. The 50% inhibition concentration values of indole-3-carboxylaldehyde, wedelolactone, luteolin and apigenin, were 34.9, 0.2, 2.4 and 9.8 μM, respectively. A formula that combined the phytocompounds in the same proportions as in the herbal extract decreased the dosage of each compound required to achieve maximal AR inhibition. In correlation with the AR suppression effect, these active compounds specifically inhibited the growth of AR-dependent PCa cells and as a combination formula they also synergistically suppressed growth in AR-dependent PCa cells. Our study has identified synergistic effects of active compounds in W. chinensis and demonstrated their potential in PCa prevention and therapy. The paradigm of multiple activities and synergism is a useful framework to investigate the therapeutic effects of whole extracts from assorted medicinal plant species.