Effect of erythorbic acid administration on activities of drug metabolic enzyme and phosphatases in guinea pigs administered an adequate amount of ascorbic acid

• Published in: Journal of Nutritional Science and Vitaminology Vol. 35; no. 2; pp. 123 - 131
• Main Authors: Suzuki, E. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life), Kurata, T, Arakawa, N
• Format: Journal Article
• Language: English
• Published: Japan Center for Academic Publications Japan 01.01.1989
• Subjects: ACID PHOSPHATASE; Acid Phosphatase - metabolism; ACIDE ASCORBIQUE; ACIDO ASCORBICO; ALKALINE PHOSPHATASE; Alkaline Phosphatase - metabolism; aniline hydrox-ylase; Aniline Hydroxylase - metabolism; Animals; ASCORBIC ACID; Ascorbic Acid - administration & dosage; Ascorbic Acid - pharmacology; Ascorbic Acid Deficiency - enzymology; CITOCROMO P 450; COBAYA; COBAYE; CYTOCHROME P 450; Cytochrome P-450 Enzyme System - metabolism; erythorbic acid; FOIE; FOSFATASA ACIDA; FOSFATASA ALCALINA; guinea pig; GUINEA PIGS; HIGADO; LIVER; Liver - enzymology; Male; PHOSPHATASE ACIDE; PHOSPHATASE ALCALINE; serum alkaline phosphatase
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.35.123

The effect of erythorbic acid (ErA) administration on activities of liver aniline hydroxylase, liver acid phosphatase, and serum alkaline phosphatase, and the content of liver cytochrome P-450 was studied to determine whether or not ErA would affect the availability of ascorbic acid (AsA) in normal and AsA-deficient guinea pigs. In experi-ment I, changes of the enzyme activities and liver cytochrome P-450 content in the guinea pigs administered AsA and/or ErA and sacrificed on days 4, 10, 16, and 30 were examined. Moreover, in experiment II, after 16 days of depletion of AsA, the guinea pigs were administered AsA and/or ErA. These animals were sacrificed on days 0, 4, and 20 of the repletion period, after which the activities of drug metabolic enzyme and phos-phatases and content of cytochrome P-450 during recovery were observed. The enzyme activities and cytochrome P-450 content of AsA-supplemented guinea pigs were similar to those of ErA-supplemented animals and also similar to those of both AsA and ErA-supplemented guinea pigs throughout the experimental period. During the repletion of the AsA-depleted guinea pigs, there were no significant differences in these enzyme activities and cytochrome P-450 content among the animals administered AsA and/or ErA. These results suggested that ErA adminis-tration may not affect the AsA availability in the guinea pigs.