The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis

• Published in: Bioscience, biotechnology, and biochemistry Vol. 74; no. 7; pp. 1386 - 1390
• Main Authors: INAGAKI, Mizuho, NAGAI, Sayaka, YABE, Tomio, NAGAOKA, Satoshi, MINAMOTO, Nobuyuki, TAKAHASHI, Takeshi, MATSUDA, Tsukasa, NAKAGOMI, Osamu, NAKAGOMI, Toyoko, EBINA, Takusaburo, KANAMARU, Yoshihiro
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Animals; Biological and medical sciences; bovine milk; Cattle; Cell Line; diarrhea; Epithelial Cells - drug effects; Epithelial Cells - virology; Fundamental and applied biological sciences. Psychology; Gastroenteritis - prevention & control; Gastroenteritis - virology; Human rotavirus; Humans; lactophorin; Membrane Glycoproteins - pharmacology; Mice; Milk Proteins - chemistry; Milk Proteins - pharmacology; PAS6/7; Peptide Fragments - pharmacology; Rotavirus - drug effects; Rotavirus - physiology; Rotavirus Infections - prevention & control; Virus Replication - drug effects; Cell culture; PAS6/7; Rotavirus; Bovine; Human rotavirus; Gastroenteritis; lactophorin; Diarrhea; bovine milk; Fragment; Reoviridae; Virus; Prevention; Vertebrata; Mammalia; Epithelial cell; Replication; Artiodactyla; Inhibition; Ungulata; Milk
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.100060

Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-M r glycoprotein fraction (F1) from cow's milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 °C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.