effect of age and non-steroidal anti-inflammatory drugs on human intestinal microbiota composition

• Published in: British journal of nutrition Vol. 103; no. 2; pp. 227 - 234
• Main Authors: Mäkivuokko, Harri, Tiihonen, Kirsti, Tynkkynen, Soile, Paulin, Lars, Rautonen, Nina
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 28.01.2010
• Subjects: Actinobacteria; Adult; age; Aged; Aged, 80 and over; Ageing; Aging; Aging - physiology; analysis; Anti-inflammatory agents; Anti-Inflammatory Agents, Non-Steroidal; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Bacteria; Biological and medical sciences; Butyrates; Butyrates - metabolism; chemistry; Clostridium; Cytosine; Cytosine - analysis; Digestive system; Digestive System Diseases; Digestive System Diseases - drug therapy; DNA, Ribosomal; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; drug effects; drug therapy; elderly; Feeding. Feeding behavior; Female; Firmicutes; Fundamental and applied biological sciences. Psychology; genetics; Guanine; Guanine - analysis; Humans; intestinal microorganisms; intestinal mucosa; Intestines; Intestines - drug effects; Intestines - microbiology; Intestines - physiology; isolation & purification; Lactobacillus; Lactobacillus - drug effects; Lactobacillus - isolation & purification; Laxatives; Laxatives - therapeutic use; Male; Mental Disorders; Mental Disorders - drug therapy; metabolism; microbial ecology; microbiology; Microbiota; molecular sequence data; Nervous System Diseases; Nervous System Diseases - drug therapy; Non-steroidal anti-inflammatory drugs; nonsteroidal anti-inflammatory agents; Nonsteroidal anti-inflammatory drugs; nucleotide sequences; Older people; Percentage guanine+cytosine; pharmacology; physiology; Ruminococcus; senescence; therapeutic use; Urologic Diseases; Urologic Diseases - drug therapy; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Young Adult; Young adults; Human; Drug; Senescence; Digestive system; Ageing; Gut; Microflora; Non-steroidal anti-inflammatory drugs; Non steroidal antiinflammatory agent; Percentage guanine + cytosine; Vertebrata; Microbiota; Mammalia; Age
• ISSN: 0007-1145; 1475-2662; 1475-2662
• DOI: 10.1017/S0007114509991553

Ageing has been suggested to cause changes in the intestinal microbial community. In the present study, the microbiota of a previously well-defined group of elderly subjects aged between 70 and 85 years, both non-steroidal anti-inflammatory drugs (NSAID) users (n 9) and non-users (n 9), were further compared with young adults (n 14) with a mean age of 28 years, by two DNA-based techniques: percentage guanine+cytosine (%G+C) profiling and 16S rDNA sequencing. Remarkable changes in microbiota were described with both methods: compared with young adults a significant reduction in overall numbers of microbes in both elderly groups was measured. Moreover, the total number of microbes in elderly NSAID users was higher than in elderly without NSAID. In 16S rDNA sequencing, shifts in all major microbial phyla, such as lower numbers of Firmicutes and an increase in numbers of Bacteroidetes in the elderly were monitored. On the genus level an interesting link between reductions in the proportion of known butyrate producers belonging to Clostridium cluster XIVa, such as Roseburia and Ruminococcus, could be demonstrated in the elderly. Moreover, in the Actinobacteria group, lower numbers of Collinsella spp. were evident in the elderly subjects with NSAID compared both with young adults and the elderly without NSAID, suggesting that the use of NSAID along with age may also influence the composition of intestinal microbiota. Furthermore, relatively high numbers of Lactobacillus appeared only in the elderly subjects without NSAID. In general, the lowered numbers of microbial members in the major phyla, Firmicutes, together with changes in the epithelial layer functions can have a significant effect on the colon health of the elderly.