Determination of vitamin B6 derivatives in foods and biological materials by reversed-phase HPLC

• Published in: Journal of Nutritional Science and Vitaminology Vol. 35; no. 3; pp. 171 - 180
• Main Authors: Toukairin, O.T. (Gifu Univ. (Japan). Faculty of Agriculture), Sakamoto, E, Hirose, N, Mori, M, Itoh, T, Tsuge, H
• Format: Journal Article
• Language: English
• Published: Japan Center for Academic Publications Japan 1989
• Subjects: Animals; BIOASSAYS; Biological Assay; CHEMICAL COMPOSITION; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS; Chromatography, High Pressure Liquid - methods; comparison of B6 contents by HPLC and bioassay; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CROMATOGRAFIA LIQUIDA ALTA PRESION; DOSAGE BIOLOGIQUE; ENSAYO BIOLOGICO; Food Analysis - methods; Food Preservation - methods; Freezing; HIGH PRESSURE LIQUID CHROMATOGRAPHY; HPLC analysis of vitamin B6; PIRIDOXINA; PYRIDOXINE; Pyridoxine - analogs & derivatives; Pyridoxine - analysis; Rats; Saccharomyces - growth & development; vitamin B6 contents in food
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.35.171

Through use of a simplified analyzing system, seven vitamin B6 derivatives were determined with a satisfactory sensitivity and precision. This system consisted of a single reversed-phase ODS column with a fluorescence detector employing an isocratic solvent system. Each vitamin B6 derivative in some foods and biological materials was determined, based on the measurement of the integrated peak area. The data obtained by this method were compared with those obtained from a bioassay by Saccharomyces uvarum ATCC 9080, after acid hydrolysis of these materials.