Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella typhimurium

• Published in: Biosensors & bioelectronics Vol. 51; pp. 195 - 200
• Main Authors: Freitas, M., Viswanathan, S., Nouws, H.P.A., Oliveira, M.B.P.P., Delerue-Matos, C.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.01.2014; Elsevier
• Subjects: Animals; Antibodies, Immobilized - chemistry; Biological and medical sciences; Biosensors; Biotechnology; Cadmium Compounds - chemistry; Cadmium nanocrystals; Electrochemical immunoassay; Electrochemical Techniques - methods; Ferric Compounds - chemistry; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Immunoassay - methods; Labeled detection; Limit of Detection; Magnetic nanoparticles; Magnetite Nanoparticles - chemistry; Methods. Procedures. Technologies; Milk - microbiology; Salmonella typhimurium; Salmonella typhimurium - isolation & purification; Sulfides - chemistry; Various methods and equipments; Labeled detection; Electrochemical immunoassay; Cadmium nanocrystals; Salmonella typhimurium; Magnetic nanoparticles; Cadmium; Gold; Nanoparticle; Iron; Probe; Immunosensor; Immunological method; Magnetic; Biosensor; Electrochemistry; Bacteria; Detection; Nanocrystal; Enterobacteriaceae
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2013.07.048

There is an imminent need for rapid methods to detect and determine pathogenic bacteria in food products as alternatives to the laborious and time-consuming culture procedures. In this work, an electrochemical immunoassay using iron/gold core/shell nanoparticles (Fe@Au) conjugated with anti-Salmonella antibodies was developed. The chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles is reported. Fe@Au nanoparticles were functionalized with different self-assembled monolayers and characterized using ultraviolet-visible spectrometry, transmission electron microscopy, and voltammetric techniques. The determination of Salmonella typhimurium, on screen-printed carbon electrodes, was performed by square-wave anodic stripping voltammetry through the use of CdS nanocrystals. The calibration curve was established between 1×101 and 1×106cells/mL and the limit of detection was 13cells/mL. The developed method showed that it is possible to determine the bacteria in milk at low concentrations and is suitable for the rapid (less than 1h) and sensitive detection of S. typhimurium in real samples. Therefore, the developed methodology could contribute to the improvement of the quality control of food samples. •Iron oxide/gold core/shell magnetic nanoparticles for Salmonella detection are made.•The magnetic property of the nanoparticles allows rapid separation of Salmonella.•Salmonella analysis could be performed in less than 1h.•A screen-printed carbon electrode for Salmonella detection is tested.•Application to milk samples is successfully performed.