Economical succinic acid production from cane molasses by Actinobacillus succinogenes

• Published in: Bioresource technology Vol. 99; no. 6; pp. 1736 - 1742
• Main Authors: Liu, Yu-Peng, Zheng, Pu, Sun, Zhi-Hao, Ni, Ye, Dong, Jin-Jun, Zhu, Lei-Lei
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.04.2008; Elsevier
• Subjects: Actinobacillus; Actinobacillus - metabolism; Actinobacillus succinogenes; batch fermentation; Biological and medical sciences; Biomass; Bioreactors; Biotechnology; Biotechnology - economics; Biotechnology - methods; bottles; Cane molasses; canes; carbon; cell growth; chemistry; economics; Fermentation; Food industries; Fundamental and applied biological sciences. Psychology; Gasoline; Hydrogen-Ion Concentration; Lipase; Lipase - chemistry; metabolism; methods; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Molasses; Nitrogen; Nitrogen - chemistry; Peptide Hydrolases; Peptide Hydrolases - chemistry; Solvents; Succinic acid; Succinic Acid - chemistry; sugars; sulfuric acid; Time Factors; Use and upgrading of agricultural and food by-products. Biotechnology; Succinic acid; Cane molasses; Actinobacillus succinogenes; Fermentation; Pasteurellaceae; Sugar cane(by product); Molasses; Actinobacillus; Bacteria
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2007.03.044

In this work, production of succinic acid by Actinobacillus succinogenes CGMCC1593 using cane molasses as a low cost carbon source was developed. In anaerobic bottles fermentation, succinic acid concentration of 50.6 ± 0.9 g l −1 was attained at 60 h using an optimum medium containing molasses pretreated with sulfuric acid, resulting in a succinic acid yield of 79.5 ± 1.1% and sugar utilization of 97.1 ± 0.6%. When batch fermentation was carried out in a 5-l stirred bioreactor with pretreated molasses, 46.4 g l −1 of succinic acid was attained at 48 h and faster cells growth was also observed. Fed batch fermentation was performed to minimize the substrate (sugar) inhibition effect, giving 55.2 g l −1 of succinic acid and 1.15 g l −1 h −1 of productivity at 48 h. The present study suggests that the inexpensive cane molasses could be utilized for the economical and efficient production of succinic acid by A. succinogenes.