Single-dose, Randomized, Open-label, 2-way Crossover Study of the Pharmacokinetics of Amitriptyline Hydrochloride 10- and 25-mg Tablet in Healthy Male Korean Volunteers

• Published in: Clinical therapeutics Vol. 37; no. 2; pp. 302 - 310
• Main Authors: Nam, Yunsung, PhD, Lim, Cheol-Hee, PhD, Lee, Ho Sung, MS, Chung, Su Jin, BS, Chung, Yoon Hee, PhD, Shin, Yong Kyoo, MD, PhD, Kim, Min-Gul, MD, PhD, Sohn, Uy Dong, PhD, Kim, Hyoung-Chun, PhD, Jeong, Ji Hoon, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2015; Elsevier Limited
• Subjects: Administration, Oral; Adult; Amitriptyline - administration & dosage; Amitriptyline - adverse effects; Amitriptyline - pharmacokinetics; amitriptyline hydrochloride; antidepressant; Antidepressive Agents, Tricyclic - administration & dosage; Antidepressive Agents, Tricyclic - adverse effects; Antidepressive Agents, Tricyclic - pharmacokinetics; Area Under Curve; Asian Continental Ancestry Group; Biological Availability; Blood & organ donations; Blood pressure; Chromatography, High Pressure Liquid; Consent; Cross-Over Studies; Drug dosages; Drug therapy; Etravil; Fasting - metabolism; FDA approval; Healthy Volunteers; Humans; Internal Medicine; Laboratories; Male; Medical Education; pharmacokinetic; proportionality; Republic of Korea; Serotonin; Tablets; Tandem Mass Spectrometry; Young Adult; Republic of Korea; proportionality; pharmacokinetic; amitriptyline hydrochloride; antidepressant; Etravil
• ISSN: 0149-2918; 1879-114X
• DOI: 10.1016/j.clinthera.2014.09.010

Abstract Purpose Amitriptyline is the most widely used tricyclic antidepressant (TCA). Although amitriptyline hydrochloride 10 and 25 mg has been marketed in Korea, no data on the dose proportionality of amitriptyline in Korean subjects are available. This clinical trial was designed to evaluate and compare the relative bioavailability with regard to dose proportionality between the two marketed strengths of amitriptyline hydrochloride tablets after a single-dose, oral administration under fasting conditions in healthy, male, Korean volunteers. Methods This single-dose, randomized, open-label, 2-way crossover study was conducted in healthy male Korean subjects. Subjects were randomly assigned to 1 of 2 dose groups and received a single dose of 10 or 25 mg amitriptyline hydrochloride under fasting conditions, followed by the alternate dose in the subsequent study period. High performance liquid chromatography (HPLC)-mass spectrometry (MS)/MS detection was applied to determine plasma concentrations. Pharmacokinetic parameters were calculated, Cmax , AUClast , AUC0–∞ , t½ , and Tmax . Statistical analysis was performed for the assessment of dose proportionality. Tolerability was assessed for up to 96 hours after administration. Findings Twelve healthy Korean subjects completed this trial (mean [S D ] age, 21.7 [1.9] years; height, 174.5 [5.0] cm; and weight, 66.7 [9.4] kg). Although 4 subjects experienced a total 5 adverse events (AEs), no serious AEs were reported during the study. The mean values of Cmax and AUC were proportional to the doses of 10 and 25 mg. The Cmax , AUClast , and AUC0–∞ of amitriptyline hydrochloride 10 mg were 5.96 ng/mL, 91.35 ng·h/mL and 109.74 ng·h/mL, respectively. The Cmax , AUClast , and AUC0–∞ of amitriptyline hydrochloride 25 mg were 17.69 ng/mL, 260.68 ng·h/mL, and 296.87 ng·h/mL, respectively. Implications Our results suggest that the 2 strengths of amitriptyline hydrochloride (10 and 25 mg) exhibited linear (dose-dependent) pharmacokinetics in these healthy, male, Korean subjects. Based on these results, a predictable and linear increase in systemic exposure can be expected. ClinicalTrials.gov identifier: NCT01367080.