Wedelolactone facilitates the early development of parthenogenetically activated porcine embryos by reducing oxidative stress and inhibiting autophagy

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e13766
• Main Authors: Wang, Xin-Qin, Liu, Rong-Ping, Wang, Jing, Luo, Dan, Li, Ying-Hua, Jiang, Hao, Xu, Yong-Nan, Kim, Nam-Hyung
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 25.07.2022; PeerJ, Inc; PeerJ Inc
• Subjects: Animals; Antioxidants; Apoptosis; Autophagy; Autophagy - genetics; Cell growth; Chemicals; Developmental Biology; Embryogenesis; Embryonic development; Embryos; Environmental Impacts; Enzymes; Female; Gene expression; In vitro fertilization; Inflammation; Kelch-Like ECH-Associated Protein 1 - genetics; Medicinal plants; Medicine, Botanic; Medicine, Herbal; Microtubule-associated protein 1; Mineral oils; NF-E2-Related Factor 2 - genetics; Nrf2/ARE; Oocytes; Ovaries; Oxidative Stress; Pharmacology; Photographic industry; Polyvinyl alcohol; Porcine embryo; Proteins; Reactive oxygen species; Swine; Traditional Chinese medicine; Wedelolactone; Women’s Health; Japan; China; United States > US; Indianapolis Indiana; Wedelolactone; Oxidative stress; Nrf2/ARE; Autophagy; Porcine embryo
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.13766

Wedelolactone (WDL) is a coumaryl ether compound extracted from the traditional Chinese medicinal plant, L. It is a natural polyphenol that exhibits a variety of pharmacological activities, such as anti-inflammatory, anti-free radical, and antioxidant activities in the bone, brain, and ovary. However, its effect on embryonic development remains unknown. The present study explored the influence of WDL supplementation of porcine oocytes culture on embryonic development and the underlying mechanisms and its effect on the levels of Kelch-like ECH-associated protein 1/nuclear factor-erythroid 2-related factor 2/antioxidant response element (Keap1/Nrf2/ARE). The results showed that WDL (2.5 nM) significantly increased the blastocyst formation rate, mitochondrial activity, and proliferation ability while reducing the reactive oxygen species accumulation, apoptosis, and autophagy. These findings suggested that WDL can enhance the growth and development of early porcine embryos to alleviate oxidative stress and autophagy through regulating and microtubule-associated protein 1 light chain 3 ( ) gene expression levels.